作物学报2011，Vol.37Issue(12)：2145-2151,7.DOI:10.3724/SP.J.1006.2011.00000
小麦品种中梁21抗条锈病基因遗传分析与SSR标记定位
Genetic Analysis and Molecular Mapping of Stripe Rust Resistance Gene in Wheat Cultivar Zhongliang 21
摘要
Abstract
Wheat cultivar Zhongliang 21 displayed resistance to epidemic yellow rust races in China when tested with seven races oiPuccinia striiformis f. Sp. Tritici (Psi). To use the elite resistance to Pst in wheat breeding, we identified the resistance gene (s) in Zhongliang 21 by means of phenotypic evaluation and molecular marker technique. Seedlings of the F1, F2, and BQ generations from the cross between Zhongliang 21 (resistant) and Mingxian 169 (susceptible), as well as the parents, were tested with Pst race CYR30. The results showed that the stripe rust resistance in Zhongliang 21 was conferred by a single dominant gene, which was designated YrzhongH, temporarily. Ten simple sequence repeat (SSR) markers located on chromosome arm 5AL, Xgwml86, Xbarcl65, Xwmc795, Xbarc40, Xgwml56, Xgwm617, Xwmc415, Xbarcl51, Xwmc338, andXgwm666, were linked to YrzhongH with the nearest flanking markers ofXgwml86 andXbarc!65. The genetic distances were 7.5 cM toXgwml86 and 2.7 cM toXbarcl65. Based on chromosomal location, reactions to various pathotypes and pedigree analysis, we deduced YrzhongH is a novel resistance gene to stripe rust. Eighteen cultivars (lines) of Zhongliang series were tested with the flanking markers Xgwm 186 and Xbarc 165, and only 17% showed the same banding pattern as that in Zhongliang 21. This result suggested that 17% of Zhongliang cultivars (lines) might carry YrzhongH, indicating that this resistance gene has a potential application in wheat breeding program for strip rust resistance.关键词
中梁21/小麦条锈病/抗病基因/遗传分析/分子标记Key words
Zhongliang 21/Wheat stripe rust/Resistance gene/Genetic analysis/Molecular mapping引用本文复制引用
马东方,王海鸽,唐明双,袁喜丽,白耀博,周新力,宋建荣,井金学..小麦品种中梁21抗条锈病基因遗传分析与SSR标记定位[J].作物学报,2011,37(12):2145-2151,7.基金项目
本研究由教育部高等学校学科创新引智计划(111计划)项目(B07049)和国家公益性(农业)行业计划专项(2009003035)资助. （111计划）
