肿瘤防治研究2011，Vol.38Issue(10)：1117-1120,4.DOI:10.3971/j.issn.1000-8578.2011.10.006
siRNA抑制食管癌EC9706细胞CXCR4基因表达的实验
Initial on Expression of CXCR4 mRNA in EC9706 Cell Silenced by siRNA
何婷玉 1杨艳丽 1赵国强2
作者信息
- 1. 464000 河南信阳,信阳市中心医院病理科
- 2. 郑州大学基础医学院微生物与免疫学教研室
- 折叠
摘要
Abstract
Objective To study the inhibitory effect of small interfering RNA(siRNA) targeting CXCR4 gene in EC9706 cells. Methods We designed two siRNAs against CXCR4 gene. Two siRNAs were trans-fected into EC9706 cells with lipofectamine, respectively. The expression level of CXCR4 mRNA in each group was detected by real-time PCR ) the effect of CXCR4 on the cell cycle was observed by flow cytom-etry; the effect of CXCR4 on the growth of the cells was observed by MTT method. Results Compared with empty vector control group and untransfected group, the mRNA level of CXCR4 in siRNA trans-fected group was obviously reduced, and the growth speed of cells was lower, S phase proportion cut down in EC9706 cells transfected with pRNAT-U6. 2/Lenti- siCXl, the exercise performance of the cells cut down in siRNA transfected group cells. Conclusion RNA interference can effectively inhibit CXCR4 expression, which plays an important role in inhibiting the growth and metastasis of the tumor.关键词
CXCR4/RNA干扰/食管癌/转移Key words
CXCR4/RNA interference/Esophageal cancer/Metastasis分类
医药卫生引用本文复制引用
何婷玉,杨艳丽,赵国强..siRNA抑制食管癌EC9706细胞CXCR4基因表达的实验[J].肿瘤防治研究,2011,38(10):1117-1120,4.
