肿瘤防治研究2011，Vol.38Issue(10)：1121-1125,5.DOI:10.3971/j.issn.1000-8578.2011.10.007
蟾毒灵对裸鼠大肠癌原位移植瘤的抗肿瘤作用及其对凋亡相关基因Bcl-xL、Bax表达的影响
Anticancer Activities and Effects of Bufalin on Apoptosis-regulate Gene Bcl-xL, Bax in Orthotopic Transplantation Model of Human Colorectal Cancer in Nude Mice
王杰 1奉典旭 1陈超 1倪振华 2左青松 1陈亚峰 1王旭 1张勇 1陈腾1
作者信息
- 1. 200062 上海,上海中医药大学附属普陀医院普外科
- 2. 200062 上海,上海中医药大学附属普陀医院实验中心
- 折叠
摘要
Abstract
Objective To investigate the anticancer activity and apoptosis-regulated mechanisms of bufalin in the orthotopic transplantation model of human colorectal cancer in nude mice. Methods HCT-116 cells of human colorectal cancer were implanted into the colon to establish orthotopic transplantation tumor models of human colorectal cancer in nude mice. Sixty mice were randomly divided into five groups: NS group,5-Fu group,Low dose bufalin group(BL), Medium dose bufalin group(BM) and High dose bufalin group(BH), with 12 mice in each group. Each group was injected intraperitoneally for 7days,respectively. Six mice in each group were killed at d24 and survival time in each group was calculated. The volume of the tumor were measured and the tumor inhibiting rate were calculated. The apoptotic rate measured by TUNEL staining method, and the expression of apoptosis regulated genes Bcl-xl and Bax were detected by real-time fluorogenic quantitative polymerase chain reaction (RFQ-PCR) and Western blot in tumor tissues. Result The tumor volume of 5-Fu group and each Bufalin group were reduced significantly compared with NS group (P<0. 01),and the tumor inhibiting rate were 69. 6%,45. 6%,56. 2% and 58. 5% for group of 5-Fu,BL,BM and BH respectively. The survival time were prolonged in group BL and BM (P<0. 05). The apoptotic rate in each group of bufalin were increased significantly compared with NS group(P<0. 05). The result of RFQ-PCR and Western blot staining showed that the expression of BcI-xl was decreased both in mRNA and protein level, while the expression of Bax was increased. Conclusion Bufalin has significant anticancer activity in the orthotopic transnplantation model of human colorectal cancer in nude mice and it can induce apoptosis of transplanted tumor cells. The mechanism may be associated with the down-regulation of Bcl-xt, and up-regulation of Bax in tumor cells.关键词
蟾毒灵/裸鼠原位移植瘤/大肠癌/细胞凋亡/Bcl-xL/BaxKey words
Bufalin/Qrthotopic transplation model in nude mice/Colorectal cancer/Apoptosis/Bcl-x1/Bax分类
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王杰,奉典旭,陈超,倪振华,左青松,陈亚峰,王旭,张勇,陈腾..蟾毒灵对裸鼠大肠癌原位移植瘤的抗肿瘤作用及其对凋亡相关基因Bcl-xL、Bax表达的影响[J].肿瘤防治研究,2011,38(10):1121-1125,5.
