中国临床药理学杂志2011，Vol.27Issue(10)：770-772,792,4.
姜黄素预处理对肢体缺血再灌注肺损伤大鼠肺内炎症反应的影响
Effects of curcumin pretreatment on pulmonary inflammatory response in lung injury induced by ischemia-reperfusion of hind limbs in rats
摘要
Abstract
Objective To investigate the effects of curcumin pretreat-ment on pulmonary inflammatory response in lung injury induced by ischemia - reperfusion (I/R) of hind limbs in rats. Methods Sixty a-dult male SD rats were randomly divided into 5 groups (n = 12, each) ; sham group; model group; three contents curcumin pretreatment groups received curcumin 50, 100, 200 mg · kg-1 respectively via intraperitone-al injection 2 h before ischemia. I/R of hind limbs was produced by occlusion of bilateral femoral arteries for 2 h followed by 3 h reperfusion in model and curcumin pretreatment groups. The animals were killed at the end of 3 h reperfusion and their lungs were immediately removed for determination of wet/dry weight ( W/D ) ratio, myeloperoxidase ( MPO ) activity, tumor necrosis factor-a (TNF -a) and interleukin - 6 (IL -6 ) content The lung was also assessed for expression of nuclear factor -kB p6S (NF - kB p65) protein using Western blot analysis. Results Lung W/D ratio, MPO activity, TNF - a and IL -6 content were significantly increased in model group as compared with sham group. Lung W/ D ratio, MPO activity, TNF-a and IL-6 content were significantly attenuated in the curcumin pretreatment groups as compared with model group in a dose - dependent manner. The expression of NF - kB p65 protein was significantly lower in the curcumin pretreatment groups than in model group in a dose - dependent manner. Conclusion Curcuminpretreatment attenuates pulmonary inflammatory response caused by I/R of hind limbs in rats due to suppressing the activation of NF - kB and subsequent accumulation of neutrophils mediated by TNF - a and IL -6.关键词
姜黄素/再灌注损伤/细胞因子Key words
curcumin/ reperfusion injury/ cytokine分类
天文与地球科学引用本文复制引用
孙晓峰,杨军,赵宏..姜黄素预处理对肢体缺血再灌注肺损伤大鼠肺内炎症反应的影响[J].中国临床药理学杂志,2011,27(10):770-772,792,4.基金项目
国家自然科学基金资助项目(30600631) （30600631）
沈阳市科学技术计划基金资助项目(F10-205-1-77) （F10-205-1-77）
沈阳医学院优秀人才启动基金资助项目(20103051) （20103051）
