摘要
Abstract
Objective To study the prevalence of human papillomavirus(HPV) infection and distribution of genotypes. Discuss association of infection of HPV and HPV genotypes with age and cervical lesions. Assessment the methodology performance and clinical diagnostic performance of PCR-Reverse Dot Blot(PCR-RDB). Methods Detect HPV DNΛ and its genotypes of cervical cy-tological specimens with PCR-RDB. The subjects were 2 994 women from outpatients and the health physical examination individuals during June 2009 to September 2010 in our hospital. In 2 994 cases,including 131 cases for DNA sequencing,1 511 cases for detection of HPV genotypes and TCT synchronously. Results HPV infection rates in 2 994 females were 25. 1%. The first fifth HPV genotypes infection were HPV52 (15. 6%) , 16(14. 1%) ,58(12. 0%) , 56 (6. 6%) and 33(5. 2%). The highest HPV infection was the group of females aged below 25,HPV16 and HPV58 included. The women who infect HPV52 most were the group aged above 55,the group aged below 25 was next. The positive rates of HR-HPV were 44. 2% in ASCUS, 91. 7% in LSIL,and 93. 3% in HSIL,respectively,the genotypes of HPV58,16,56 and 31 had correlations with the degree of cervical lesions(The regression coefficients were 0. 493,1. 744 ,-0. 819 and 1. 566, respectively). HPV52 was not significant correlation with the degree of cervical lesions. With sequencing and the result of TCT≥LSIL as gold standard,for PCR-RDB in HPV and diagnosis of cervical lesions, the sensitivity,specificity,accuracy were 96. 4%and 92. 2% ,93. 4%and 84. 2% ,94. 7%and 84. 5% respectively. Conclusion The proportion of HPV infection in female is quite high and young women are more susceptible to infections. The major pathogenic types are HPV52,16 ,58, 56 and 33. HPV infection is highly correlated with cervical lesions level. Detecting HPV with PCR-RDB can fully satisfy the need of clinical testing on the methodology performance and clinical diagnostic performance in diagnosing cervical lesions.关键词
人乳头瘤病毒/基因分型/子宫颈细胞学Key words
human papillomavirus/ genotyping/ cervical cytology
