重庆医学2011，Vol.40Issue(33)：3340-3341,3345,3.DOI:10.3969/j.issn.1671-8348.2011.33.004
Cenp-E表达下调与HepG-2细胞染色体数目异常的关系研究
Study on the relationship between the low expression of Cenp-E and chromosome numerical abnormality in HepG-2
刘斌 1刘卓然 1翁亚光 2刘子杰 2蔡燕2
作者信息
- 1. 南华大学附属第二医院,湖南衡阳,421001
- 2. 重庆医科大学检验系,400016
- 折叠
摘要
Abstract
Objective To study the role and localization of Cenp-E gene in HepG-2 cell. Methods The cell cycle changes of HepG-2 cell and LO2 cell synchronized by nocodazole were detected by flow cytometry. The chromosome assay was uesd to detected the number and karyotype of chromosome of HepG-2 cell and LO2 cell. Cenp-E mRNA expressions in HepG-2 cell and LO2 cell synchronized by nocodazole were detected by RFQ-PCR. In the mean while. Cenp-E protein localization and expressions in HepG-2 cell and LO2 cell were oberved by indirect immunofluorescence. In the end,RNAi technology was used to further evaluated the function of Cenp-E in LO2 cell. Results HepG-2 cell and LO2 cell were arrested in G2/M phase synchronized by nocodazole. The chromosome assay showed that the proportion of abnormal chromosomes cell in HepG-2 cell were higher then LO2 cell. After synchronized by nocodazole,Cenp-E mRNA and protein expressions of HepG-2 cell was higher then LO2 cell. Cenp-E mRNA and protein expressions of LO2 cell decreased significantly. Conclusion The low expression of Cenp-E may be one of the important reasons of chromosome numerical abnormality in human hepatoma cells.关键词
肝肿瘤/染色体/纺锤体检查点蛋白EKey words
liver neoplasms/ chromosomes/ spindle checkpoint proteinE引用本文复制引用
刘斌,刘卓然,翁亚光,刘子杰,蔡燕..Cenp-E表达下调与HepG-2细胞染色体数目异常的关系研究[J].重庆医学,2011,40(33):3340-3341,3345,3.
