临床口腔医学杂志2011，Vol.27Issue(10)：598-602,5.DOI:10.3969/j.issn.1003-1634.2011.10.007
孕酮对人牙周膜细胞增殖及成骨分化的调控作用
Progesterone modulates the proliferation and differentiation of the human periodontal ligament cells
原工杰 1刘琳 1肖云鹤 2丁寅3
作者信息
- 1. 大连市口腔医院大连大学附属口腔医院正崎科,辽宁,大连,116002
- 2. 武汉市第五医院口腔科,湖北,武汉,430071
- 3. 第四军医大学口腔医院正崎科,陕西,西安,710032
- 折叠
摘要
Abstract
Objective: To detect the expression of progesterone receptor (PR) in human periodontal ligament cells (hPDLCs) and investigate the proliferation and bone-sparing effects of progesterone. Method: The hPDLCs were stimulated with progesterone (1,10,100 nM) for 1,2,3>4,5,6 and 7 days. MTT assay was performed to assess the effects of progesterone on cell growth curve. To quantify the effects of progesterone on osteogenic differentiation of hPDLCs. Alizarin red was performed in basic culture medium and osteogenic medium. Result: The positive signal was strongly expressed in the nuclei but weaker in cytoplasm of hPDLCs. Progesterone can stimulate the proliferation of the hPDLCs. And the PgR antagonist (RU486) can block the effect of progesterone. In both basic culture medium and osteogenic medium,progesterone activated alizarin red-positive nodules. The amount of mineralized matrix increased in the progesterone-treated group in osteogenic medium. Conclusion: Progesterone can stimulate the proliferation and differentiation of the hPDLCs.关键词
孕酮/孕激素受体人牙周膜细胞/细胞增殖/成骨分化Key words
progesterone/ progesterone receptor/ human periodontal ligament cells/ cell proliferations differentiation分类
生物科学引用本文复制引用
原工杰,刘琳,肖云鹤,丁寅..孕酮对人牙周膜细胞增殖及成骨分化的调控作用[J].临床口腔医学杂志,2011,27(10):598-602,5.
