重庆医学2011，Vol.40Issue(33)：3391-3392,3425,3.DOI:10.3969/j.issn.1671-8348.2011.33.028
微型光谱仪检测血清二氧化碳的研究
Experimental study on the detection of carbon dioxide based on dry chemistry method with micro spectrometer
摘要
Abstract
Objective To establish a new method for rapid detection of carbon dioxide based on micro spectrometer and dry reagent. Methods The dry powder carbon dioxide reagent was prepared through enzymatic method, its detection method in the micro spectrometer was explored and the performance of both the reagent and the equipment was evaluated with statistical analysis. Results The prepared dry reagents were of rarefaction structures and with good reaction solubility for 90 days after being lyophilized. The linear range of carbon dioxide was 15- 40 mmol/L,detecting at 54 6 nm wave length. The intra-assay and inter-assay coefficient variations (CV) were respectively less than 4 % and less than 6 % , with the recovery rate ranging from 98. 8 % to 105. 8 %. No obvious interference was observed in the determination results when the serum TB was less than 290. 40 μmol/L and the TG was less than 11. 20 mmol/L. The detection results by this method correlated well with that from the automatic biochemistry analyzer enzymatic method (r=0. 956). Conclusion The method is accurate and reliable in determining the serum carbon dioxide,and its process is simple and convenient to carry out. Therefore, it can be qualified as a rapid batch detection method for serum carbon dioxide.关键词
二氧化碳/微型光谱仪/干试剂Key words
carbon dioxide/ micro spectrometer/ dry reagent引用本文复制引用
傅灵媛,吴杰红,张志伦,蒲晓允..微型光谱仪检测血清二氧化碳的研究[J].重庆医学,2011,40(33):3391-3392,3425,3.基金项目
解放军总装备部预研基金(2007E056). （2007E056）
