肿瘤防治研究2011，Vol.38Issue(10)：1133-1136,4.DOI:10.3971/j.issn.1000-8578.2011.10.010
鼻咽癌Ki67表达与放疗前后18F-FDG PETCT显像相关性的动物模型及临床研究
Animal Model and Clinical Studies of Relationship between Ki67 Expression and 18F-FDG PET-CT Inmging of Nasopharyngeal Carcinoma before and after Radiotherapy
冯彦林 1杨明 1余丰文 1梁伟棠 1贺小红 1张洋 2冼伟均 1袁建伟 1张宁 2黄克敏 1袁白虹 1苏少弟 1温广华 1刘德军1
作者信息
- 1. 528000 广东佛山,中山大学附属佛山医院核医学科
- 2. 佛山市第一人民医院放疗科
- 折叠
摘要
Abstract
Objective To investigate the relationship between the expression of Ki67 and the changes in SUV-max of nasopharyngeal carcinoma before and after radiotherapy. Methods (1) Seventeen nude mice transplanted nasopharyngeal carcinoma xenografts were randomly divided into the control group, the 6Gy irradiated group and the 12 Gy irradiated group. The radiation groups underwent 18F-FDG PET-CT examination before and after radiotherapy and the expressions of Ki67 were detected by immunohistochemistry after radiation. Then the changes of the Ki67 expression and T/NT ratios in xenografts were analyzed (2) Twelve untreated patients with nasopharyngeal carcinoma underwent 18F-FDG PET-CT examination before radiotherapy, during radiation when 50 Gy was given and 1 week after radiation, respectively. Then the SUVmax of nasopharyngeal carcinoma was measured. The expression of Ki67 in patients' paraffin blocks before radiotherapy were detected by immunohistochemical method, and then the correlation between the Ki67 expression and the changes of SUV max during radiation were analyzed. Results (1) The expression of Ki67 antigen in xenografts reduced significantly with increasing radiation dose, and the expressions of Ki67 among different groups were dramatically different (F = 57. 8, P<0. 01). (2) The T/NT ratios among the different groups weredramatically different (F = 14. 0,P<0. 01). (3) The SUVmax of lesions in patients highly expressed Ki67 was significantly lower than that in patients with lower Ki67 expression when received 50Gy radiation (t = 2. 235,P<0. 05). But there were no statistical difference before radiotherapy (t = 0. 176,P>0. 05) or after 12Gy radiation(t= 1. 106,P>0. 05). Conclusion The Ki67 expression is an important factor contributing to the FDG uptake of nasopharyngeal carcinoma. And the expression of Ki67 before radiotherapy may have an important impact on the reduction of SUVmax of nasopharyngeal carcinoma during radiation therapy.关键词
鼻咽肿物/放射疗法/Ki67/体层摄影术,发射型计算机/裸鼠Key words
Nasopharyngeal neoplasm/Radiotherapy/Ki67/Tomography, emission- computed/Nude mice分类
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冯彦林,杨明,余丰文,梁伟棠,贺小红,张洋,冼伟均,袁建伟,张宁,黄克敏,袁白虹,苏少弟,温广华,刘德军..鼻咽癌Ki67表达与放疗前后18F-FDG PETCT显像相关性的动物模型及临床研究[J].肿瘤防治研究,2011,38(10):1133-1136,4.
