四川医学2011，Vol.32Issue(9)：1336-1338,3.
宫颈永生化细胞和癌细胞胞膜差异蛋白质组学研究
Comparative Proteomics analysis of membranous proteins of immortalized cervical cell and cervical cancer cell
宋大萍 1赵涌2
作者信息
- 1. 绵阳市第三人民医院病理科,四川绵阳621000
- 2. 重庆医科大学病理教研室,重庆400016
- 折叠
摘要
Abstract
Objective To identify differentially expressed membranous protein spots of from human immortalized endocer-vical cell ( H8 ) and cervical cancer cell ( Caski) associated with cervical carcinogenesis by two-dimensional electrophoresis (2-DE) analysis. Methods The membranous proteins of H8 cell and Caski cell were separated by means of two-dimensional gel electrophoresis, then the selected differential protein spots were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and SW1SS-PROT database. Results 13 differentially expressed proteins spots from these membranous proteins in H8 cells and Caski cells were further identified by MALDI-TOF-MS analysis and 3 of them were successfully identified and characterized. LRC8D(Leucine-rich repeat-containing protein 8D) 、EDAR( Tumor necrosis factor receptor superfamily member EDAR precursor)、Metaxin-l were significantly up-regulated in membranous proteins in H8 cells. Conclusion There differential expressions of membranous proteins of may be valuable for studying mechanisms of cervical carcinogenesis, or as diagnostic markers and therapeutic targets for cervical cancer.关键词
H8细胞/Caski细胞/差异蛋白质组/2-DE/质谱Key words
H8 cell/ caski cell/ comparative proteomics/ two-dimensional gel electrophoresis / MALDI-TOF-MS分类
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宋大萍,赵涌..宫颈永生化细胞和癌细胞胞膜差异蛋白质组学研究[J].四川医学,2011,32(9):1336-1338,3.
