作物学报2011，Vol.37Issue(8)：1351-1359,9.DOI:10.3724/SP.J.1006.2011.01351
野生大豆转录因子GsNA C20基因的分离及胁迫耐性分析
Isolation and Tolerance Analysis of GsNAC20 Gene Linked to Response to Stress in Glycine soja
摘要
Abstract
Abiotic stresses, such as salt and drought, affect plant growth, development and reduce crop yield. Isolation of a key regulatory gene linked to response to abiotic-stress and identification of the genes function are urgently needed. Glycine soja is an excellent material to isolate abiotic stress-related genes because of its high stress tolerance. Plant-specific transcription factor NAC (NAM, ATAF1/2, CUC2) proteins play essential roles in many biological processes such as development, senescence, morphogenesis, and stress signal transduction pathways. It has become a new research focus in the abiotic-stress field. Based on that, we screened a new NAC gene from Glycine soja by yeast one hybrid, which has 99% similarity with NAC20 of Glycine max (EU44O353.1), named as GsNAC20. GsNAC20 had typical NAC DNA-binding domain at the N-terminal and transcription activation region at the C-terminal. It can bind to MYB1AT element (the core sequence: AAACCA) in vitro, but no transcriptional activation activity in the yeast assay system, which was consistent with GmNAC20. Localization of GsNAC20 protein was analyzed by transient expression in tobacco epidermis cells and the result showed that GsNAC20 was localized in nucleus. Semi-quantitative RT-PCR showed the expression level of GsNAC20 was induced by drought, low temperature and salt stresses, but there existed difference between leaf and root in G soja. Arabidopsis thaliana plants overexpressing GsNAC20 showed higher sensitivity under salt stress. All results showed that GsNAC20 perhaps is a new member of NAC family in G soja, and is closely related to salt and drought stresses, so it can either be used as a new resource in gene engineering on stress tolerance or be further studied to provide more information for the researches on the mechanism of stress tolerance in plant.关键词
野生大豆/转录因子/GsNA C20/非生物胁迫Key words
Glycine soja/ Transcription factor/ GsNAC20/ Abiotic stress引用本文复制引用
才华,朱延明,李勇,柏锡,纪巍,千冬冬,孙晓丽..野生大豆转录因子GsNA C20基因的分离及胁迫耐性分析[J].作物学报,2011,37(8):1351-1359,9.基金项目
本研究由黑龙江省教育厅科技项目(11521021),黑龙江省博士后基金(LBH-Z08253),国家重大基础研究前期专项(2003CCA03500)和国家自然科学基金项目(30471059)资助. （11521021）
