水生生物学报2011，Vol.35Issue(4)：557-564,8.DOI:10.3724/SP.J.1035.2011.00557
黄喉拟水龟转铁蛋白基因的克隆以及表达特征分析
CLONING AND EXPRESSION PATTERN ANALYSIS OF TRANSFERRIN GENE IN YELLOW POND TURTLE
摘要
Abstract
Transferrin plays an important role in the iron metabolism and participates in the immune response after infected by the bacterial. The full-length Tf genomic cDNA sequence was cloned, and the characteristics of Tf gene sequence were analyzed. Sequencing analysis showed that Tf of the Yellow pond turtle constituted a single peptide chain by two similar domains, each domain consisted of two subunits, which interacted each other and formed a deep, hydro-philic iron binding sites. The Tf genomic DNA contained 17 exons and 16 introns, and the structure of Tf gene similar to other vertebrates, which showed that the Tf gene structure of the Yellow Pond Turtle was conserved. Homologous analysis showed that the homology of the Tf gene among the Yellow Pond Turtle, birds and reptiles was about 75%-97%; the homology of the Tf gene between the Yellow Pond Turtle and mammalian 65%-75%; the homology of the Tf gene among the Yellow Pond Turtle, Xenopus laevis and other amphibians was very little. Quantitative PCR results showed that the expression of MaTf in the liver, spleen, kidney and heart tissues increased after infected by Serra-tia marcescens in different time, and the characteristics was similar with other animals which were infected by a pathogen.关键词
黄喉拟水龟；转铁蛋白；序列分析；组织表达Key words
Yellow pond turtle/ Transferrin/ Sequence analysis/ Tissue-specific expression分类
生物科学引用本文复制引用
高明英,朱新平,赵密,史燕,赵建..黄喉拟水龟转铁蛋白基因的克隆以及表达特征分析[J].水生生物学报,2011,35(4):557-564,8.基金项目
国家重点基础研究发展计划项目(2004CB117401)；广东省科技兴渔项目(B200701A06)资助 （2004CB117401）
