水生生物学报2011，Vol.35Issue(4)：572-577,6.DOI:10.3724/SP.J.1035.2011.00572
斑马鱼Zar1和Zar1-like基因的克隆及其表达分析
MOLECULAR CLONING AND EXPRESSION PATTERN OF ZAR1 AND ZAR1L IN THE ZEBRAFISH
摘要
Abstract
Maternal mRNAs, which are expressed in oocytes, play an important role in the success of early embryonic development. Zygote arrest 1 (Zarl) and Zarl-like (ZarlL) are the oocyte-specific maternal genes that function at the oocyte-to-embryo transition in mouse. Zarl and ZarlL are conserved in evolution, but the expression patterns of Zarl and ZarlL are diverse in animal kingdom. In this experiment, the full length of ZarlL cDNA was cloned from the ze-brafish ovary by RACE. The expression patterns of Zarl and ZarlL in different tissues, developing oocytes and em-bryogenesis were checked by RT-PCR. The full-length cDNA of ZarlL was 1146 bp, which comprised a 20 bp 5' untranslated region (UTR), a 190 bp 3'-UTR; a 936 bp open reading frame (ORF) encoding a 311 amino acids peptide. Structurally, the ZarlL protein contained an atypical PHD motif (plant homeo domain) and two C4 type zinc finger in the C-terminal, and showed 74% homology to known counterparts from mammals and birds. In gene structure, Zarl and ZarlL had four exons, each of the two genes exons were relatively similar in size. It showed that zebrafish Zarl and ZarlL gene belonged to two different genes, and it might be the ancestors of the same source gene duplication and evolution. Zarl and ZarlL were expressed at a similar pattern, and both of them were ovary specific. The expressions of Zarl and ZarlL did not change during oogenesis. Both Zarl and ZarlL mRNA were maternal deposited, and the expression levels of both genes were high in the early embryos till gastrulation. A significant decrease of ZarlL occurred at gastrula, while Zarl decreased apparently at neurula. However, both Zarl and ZarlL were expressed in the whole process of embryonic development till hatching. The consistency expression level of Zebrafish Zarl and ZarlL implied that the function of the two genes were basically the same. The results suggested that Zarl and ZarlL were maternal factors for mid-blastula transition, and Zarl and ZarlL might have other important roles during embryonic development.关键词
斑马鱼；Zar1；Zar1L；母体-合子转变；基因克隆；表达模式Key words
Zebrafish/ Zarl/ ZarlL/ Maternal-effect gene/ Gene cloning/ Expression pattern分类
生物科学引用本文复制引用
江文波,赵冬梅,胡炜,赵浩斌..斑马鱼Zar1和Zar1-like基因的克隆及其表达分析[J].水生生物学报,2011,35(4):572-577,6.基金项目
淡水生态与生物技术国家重点实验室开放基金；湖北省遗传调控与整合生物学重点实验室开放基金资助 （）
