水生生物学报2011，Vol.35Issue(4)：666-671,6.DOI:10.3724/SP.J.1035.2011.00666
人工感染三角帆蚌病毒性疾病的组织病理学观察
HISTOPATHOLOGICAL EXAMINATION OF BIVALVE MUSSEL HYRIOPSIS CUMINGII LEA ARTIFICIALLY INFECTED BY VIRUS
摘要
Abstract
The pathogen Hyriopsis cumingii Plague Virus isolated from the diseased H. cumingii Lea has been artificially transferred to the healthy H. cumingii Lea. The infected H. cumingii Lea has displayed some typical pathological changes. Observations by histopathology have showed that most of organs including the digestive gland, stomach and intestine are the primary targets of the plague virus. Lesions have been observed, accompanied with a number of empty areas, cell vacuolization and some cells falling-off. Arenavirus-like particles have been detected by using transmission electron microscopic (TEM) examination on investigated organs of the artificially infected bivalve mussel Hyriopsis cumingii. Virions are spherical with a size of 120 nm in diameter, and the outer layer of the intact arenavirus-like particles have small protuberance and moderate electron-dense nucleicapsid. Under electronic microscope, the most obvious cellular pathologic feature is the large vacuole areas of cytoplasm, and there is the reduction or devoid of organelles in the cytoplasm. Varying degrees of denaturalization and necrosis have been found in the intestinal mucosa epithelial cells.We have also observed that in cytopathic cells of digestive gland, the nuclei has been discovered to be amorphous and hypertrophied. There are denaturalization and necrosis in the epithelial cell of gill and the nucleus membrane of granulocytes have been dissolved.关键词
三角帆蚌；砂样病毒；显微结构；超微结构；组织病理学Key words
Hyriopsis cumingii Lea/Arenavirus-like/Microstructure/Ultrastructure/Histopathology分类
农业科技引用本文复制引用
钟蕾,肖调义 ,黄倢,戴良英,刘晓燕..人工感染三角帆蚌病毒性疾病的组织病理学观察[J].水生生物学报,2011,35(4):666-671,6.基金项目
The Science and Technology Support Projects of China (Grant No.2007BAD87B11) （Grant No.2007BAD87B11）
the Science and Technology Research Projects,Hunan Province,Grant No.06NK3049) （）
