中国药房2011，Vol.22Issue(37)：3523-3525,3.
颗粒定置琼脂基空白对照法在微生物限度检查中的应用
Application of Particle Fixation Cultivate Dish Blank Contrast Method in Microbial Limit Test
张娅琴 1张雅稚 1李家琪1
作者信息
- 1. 华中科技大学同济医学院附属襄阳医院,襄阳市441021
- 折叠
摘要
Abstract
OBJECTIVE: To study a kind of simple, rapid, accurate test method to identify the microbial limits detection of bacterial colonies and drug granules. METHODS: Particle fixation cultivate dish was prepared as blank control using agar powder and microbial limit detection of samples was conducted. The number of blank control colonies was subtracted from colonies of nutrition medium to obtain the actual number of bacterial colonies; standard bacterial growth test of prepared particle fixation cultivate dish was conducted; validation of standard bacterial growth test for self-made samples of our hospital was carried out and it was compared with microscopic examination counting process stated in Chinese Pharmacopeia. RESULTS: No standard bacterial growth was observed on the particle fixation cultivate dish. The growth test of self-made test solution of our hospital was in accordance with the result of microscopic examination to the extent of 100%. The time spending on determination was 0.40 min for the former and 14.12 min for the latter. CONCLUSION: The result of microbial limit test using particle fixation cultivate dish as blank control is same as that of microscopic examination counting. The method is proven to be scientific, simple, rapid and accurate.关键词
微生物限度检查/颗粒定置琼脂基/空白对照法/显微镜检法/菌落/药物颗粒Key words
Microbial limit test/ Particle fixation cultivate dish/ Blank contrast method/ Microscopy identify method/ Bacterial colonies/ Drug granules分类
医药卫生引用本文复制引用
张娅琴,张雅稚,李家琪..颗粒定置琼脂基空白对照法在微生物限度检查中的应用[J].中国药房,2011,22(37):3523-3525,3.
