作物学报2011，Vol.37Issue(8)：1497-1502,6.DOI:10.3724/SP.J.1006.2011.01497
普通小麦品种小偃54中α/β-醇溶蛋白编码基因的克隆与序列分析
Cloning and Sequence Analysis of α/β-gliadin Genes from Common Wheat Variety Xiaoyan 54
摘要
Abstract
We developed a method for identifying the genomic BAC clones containing the a/β-gliadin gene sequences of common wheat (Triticum aestivum L.). Using this method, nine unique positive clones harboring a/β-gliadin genes were obtained from the BAC library of the elite wheat variety Xiaoyan 54. From these BAC clones, 17 distinct a/β-gliadin gene sequences were isolated, 12 of which were pseudogenes because of the presence of one or more premature stop codons in their open reading frame (ORF). The remaining five genes (tentatively designated Gli-Xy54-1, Gli-Xy54-2, Gli-Xy54-3, Gli-Xy54-7, and Gli-Xy54-13) possessed intact ORF. Their deduced protein sequences contained 291, 310, 311, 287, and 317 amino acid residues, respectively, and resembled highly the primary structure of previously reported a/β-gliadins. The presence of celiac disease inducing epitopes and the length of the poly-glutamine repeats in the five deduced a/β-gliadins were analyzed. Based on these data, it was suggested that Gli-Xy54-1 might be located on chromosome 6A, Gli-Xy54-2, Gli-Xy54-3, and Gli-Xy54-13 on chromosome 6B, and Gli-Xy54-7 on chromosome 6D. This proposition was supported by phylogenetic analysis of the gliadin gene sequences isolated here and previously. As far as we know, this work represents the first report on the identification of BAC clones containing the a/β-gliadin genes from common wheat. The genes isolated here may facilitate further investigations on the composition, expression and function of a/β-gliadin genes in common wheat.关键词
普通小麦/α/β-醇溶蛋白/BAC文库筛选/基因克隆Key words
Common wheat/ a/β-gliadin/ BAC library screening/ Gene cloning引用本文复制引用
张晓霞,焦浈,董振营,李世明,王燃,凌宏清,秦广雍,王道文..普通小麦品种小偃54中α/β-醇溶蛋白编码基因的克隆与序列分析[J].作物学报,2011,37(8):1497-1502,6.基金项目
本研究由植物细胞与染色体工程国家重点实验室开放课题(PCCE-2008-KF-02)和河南省重大科技专项(081100110500)资助. （PCCE-2008-KF-02）
