中药材2011，Vol.34Issue(9)：1351-1355,5.
高良姜道地药材的高相液相色谱指纹图谱研究
Study on HPLC Fingerprint of Alpinia officinarum
摘要
Abstract
Objective: To establish the chromatography fingerprint of Alpinia offkinamm by HPLC. Methods; An optimum HPLC conditions which were obtained under the assessment of LC-MS were as follows; Shim-pack VP-ODS column(2. 0 mm x 250 mm, 5ujn) , 0. L%Hac aqueous solution as phase A, 15% Acetonitrile:40% Methanol:45%Tetrafuran as phase B, the flow rate was 0. 20 mi/min, column temperature was 35t and UV detector was set at 280 ran. Results; The HPLC fingerprint of Alpinia officinanun was established,the consensus 10 peaks and their relative retention times along with the ranges of relative area were determined. Conclusion ; The method is reliable and stable and can be used for the quality control and identification of Alpinia officinarum.关键词
高良姜/指纹图谱/高效液相色谱Key words
Alpinia officinarum Hance/ Fingerprint/ HPLC分类
医药卫生引用本文复制引用
邓亦峰,冯丽娜,罗辉..高良姜道地药材的高相液相色谱指纹图谱研究[J].中药材,2011,34(9):1351-1355,5.基金项目
广东省建设中医药强省科研基金资助项目(No.2060044) （No.2060044）
