首页|期刊导航|中德临床肿瘤学杂志（英文版）|Research of vitamin E succinate combined with paclitaxel on the apoptosis of Her-2 over-expressing breast cancer cells
中德临床肿瘤学杂志（英文版）2011，Vol.10Issue(11)：661-665,5.DOI:10.1007/s10330-011-0872-x
Research of vitamin E succinate combined with paclitaxel on the apoptosis of Her-2 over-expressing breast cancer cells
Research of vitamin E succinate combined with paclitaxel on the apoptosis of Her-2 over-expressing breast cancer cells
摘要
Abstract
Objective: The aim of this study was to detect apoptosis rates of Her-2 overexpression breast cancer cells, which were administrated with vitamin E succinate (VES) combined with paclitaxel at different dosages, or administrated alone; to discuss the mechanism of their actions. Methods: Using immunohistochemical method to detect Her-2 expression of MDA-MB-453 cells. Using TUNEL assay to detect apoptosis rates of MDA-MB-453 cells, with the concentrations at 10, 20 mg/L of VES and 50, 100 nmol/L of paclitaxel, and also combined together for 24 or 48 h. Then compared apoptosis action of various combinations. Results: The expression rate of 95% Her-2 was interval (63.32%, 69.60%); VES and paclitaxel both induced apoptosis of MDA-MB-453 cells, and it is dose to time dependence. It was strongest in apoptosis at 10 mg/L VES and 100 nmol/L paclitaxel in MDA-MB-453 cells 48 h later. Conclusion: VES and paclitaxel both induced apoptosis of MDA-MB-453 cells. It is stronger when the two drugs are administrated together. The mechanism is probably related to reduction of bcl-2 expression, so as to be more sensitive to paclitaxel. Synergistic effect is also possible for the two drugs influence tumor cells in different growing phases.关键词
vitmian E succinate (VES)/paclitaxel/breast cancer/Her-2/apoptosisKey words
vitmian E succinate (VES)/paclitaxel/breast cancer/Her-2/apoptosis引用本文复制引用
Li Li,Tingting Yan,Yan Zhao,Qiuying Jiang..Research of vitamin E succinate combined with paclitaxel on the apoptosis of Her-2 over-expressing breast cancer cells[J].中德临床肿瘤学杂志（英文版）,2011,10(11):661-665,5.基金项目
Supported by a grant from the Educational Department of Province Hei-lonjiang (No.1521185). （No.1521185）
