生物加工过程2011，Vol.9Issue(4)：1-5,5.DOI:10.3969/j.issn.1672-3678.2011.04.001
响应面法优化松茸发酵产多糖的培养条件
Optimization of fermentation conditions for polysaccharide of Tricholoma matsutake using response surface methodology
摘要
Abstract
Response surface methodology was adopted to optimize fermentation conditions for polysac-charide of Tricholoma matsutake. Firstly, based on the results of carbon sources and nitrogen source experiments, a Plackett-Burman design was used to investigate the effects of different factors on production of polysaccharide, and three significant factors were corn flour, soybean meal, and KH2PO4. The path of steepest ascent was used to define optimal response region for these three factors. Optimal level combinations of factors were obtained through Box-Behnken design. The optimal fermentation conditions for polysaccharide of Tricholoma matsutake were as follows; corn flour 4. 54% , soybean meal 4. 96% , KH2PO4 0. 15% , MgSO4 -7H2O 0. 05% , VB1 0. 001% , initial pH 5.5, rotation speed 180 r/min, and fermentation time 10 d. Under optimal fermentation conditions, the yield of polysaccharide reached 5. 97 g/L.关键词
实验设计/发酵/多糖/松茸Key words
experimental design/ fermentation/ polysaccharides/ Tricholoma matsutake分类
化学化工引用本文复制引用
郝继伟,邱奉同..响应面法优化松茸发酵产多糖的培养条件[J].生物加工过程,2011,9(4):1-5,5.基金项目
临沂大学社会立项课题项目(2010ly0401) （2010ly0401）
