生物加工过程2011，Vol.9Issue(4)：31-34,4.DOI:10.3969/j.issn.1672-3678.2011.04.007
油橄榄叶中橄榄苦苷的分离纯化
Separation and purification of oleuropein from olive leaves
摘要
Abstract
Extraction, purification and separation of oleuropein in olive leaf extract were studied by 6 different macroporns resins. Macroporous absorption resins were compared on adsorption and desorption performances. To achieve an optimal purification of oleuropein, the adsorption and desorption characteristics of the best resin were investigated. The optimal column separation conditions of macroporous resin D101 were as follows: the mass concentration of oleuropein in the liquid was 41. 06 mg/mL, the flow rate was 5 mL/min, 50% aqueous ethanol solution was effective solvent for oleuropein desorption, the volume of eluent was 260 mL. The content of oleuropein was more than 55% , the oleuropein was obtained by purification again using Sephadex, and 76% yield was obtained.关键词
柱层析法/大孔吸附树脂/橄榄苦苷Key words
column chromatography/ macroporous resin/ oleuropein分类
医药卫生引用本文复制引用
傅珊,万文倩,杨文革,胡永红..油橄榄叶中橄榄苦苷的分离纯化[J].生物加工过程,2011,9(4):31-34,4.基金项目
江苏省高校自然科学基础研究项目(08KJA530002) （08KJA530002）
