生物加工过程2011，Vol.9Issue(4)：58-63,6.DOI:10.3969/j.issn.1672-3678.2011.04.013
适配子纳米生物传感器在青霉素检测中的应用
Application of aptamer nano-biosensor in penicillin detection
摘要
Abstract
Porous silicon (PS) was made from silicons by a pulsed current etching method. Three methods were tested to choose the best way of immobilizing penicillin aptamer on glass sheet, called the cova-lent bonding method, ion-absorbing method, and APTES-modified glutaraldehyde cross-linking method. After penicillin aptamer was immobilized on the modified PS, the impedances were determined and compared by AC impedence method under conditions of adding and without adding penicillin. Then, an equivalent circuit was established for impedance fitting. The Nyquist plots of PS sensor could prove that the aptamer was immobilized and the nano-biosensor established. The linear range of the sensor was 0. 05 -0. 20 mg/L. The sensitivity limit was 0. 05 mg/L.关键词
适配子/生物传感器/多孔硅Key words
aptamer/ biosensor/ porous silicon分类
医药卫生引用本文复制引用
孙坤,张娟琨,徐培东,王明伟,林青,徐锦锋,宋可..适配子纳米生物传感器在青霉素检测中的应用[J].生物加工过程,2011,9(4):58-63,6.基金项目
科技部资助项目(2009CJA10047) （2009CJA10047）
天津市科委资助项目(07ZHXHNC04500,09ZCZDSF04200) （07ZHXHNC04500,09ZCZDSF04200）
