中国药理学通报2011，Vol.27Issue(12)：1678-1682,5.DOI:10.3969/j.issn.1001-1978.2011.12.012
依普利酮抑制细胞增殖拮抗急性环孢素A肾损伤的研究
Inhibitory effect of eplerenone on cell proliferation in acute cyclosporine nephrotoxicity
摘要
Abstract
Aim To investigate the inhibitory effect of mineralocorticoid receptor blocked eplerenone on cell proliferation in acute cyclosporine nephrotoxicity. Methods Acute cyclosporine nephrotoxicity in mice was induced by cyclosporine A ( CsA ) with 100 mg · kg-1 · d-1 orally and treated with eplerenone 100 mg· kg -1 · d-1. The blood and kidneys were harvested on the 10th day. Serum aldosterone was detected with radioimmunoassay. Proliferaion cell nuclear antigen ( PCNA ) and fibronectin ( FN ) were measured with immunohistochemistry. PCNA, FN and TGF-β1 were detected with RT-PCR and western bolt. Results Al-dosterone level in blood was increased in CsA treated mice ( 318. 83 ± 95. 72 )ng · L-1 than that in control mice( 82.05 ± 23. 04 ) ng · L-1 and inhibited by eplerenone ( 149. 50 ±36. 20 )ng · L-1. PCNA positive cells and FN increased in CsA group with immunohisto-chemistry. Expression of PCNA,FN and TGF-β1 were up-regulated in CsA treated mice by RT-PCR and west-ern blot and down-regulated by eplerenone. Couclu-sion Eplerenone has the role of reducing secretion of aldosterone, inhibiting the cell proliferation thus reducing the renal damage induced by CsA.关键词
依普利酮/环孢素A肾毒性/盐皮质激素受体阻断剂/增殖细胞核抗原/细胞增殖/醛固酮Key words
eplerenone/ acute cyclosporine nephrotox-icity/ mineralocorticoid receptor blockade/ proliferaion cell nuclear antigen/ cell proliferation/ aldosterone分类
医药卫生引用本文复制引用
孙东云,许庆友,王香婷,王霞,王筝,王聪慧,穆胜宇,下则达雄,藤田敏朗..依普利酮抑制细胞增殖拮抗急性环孢素A肾损伤的研究[J].中国药理学通报,2011,27(12):1678-1682,5.基金项目
河北省优秀专家出国培训资助项目(2008年) （2008年）
