中国药理学通报2011，Vol.27Issue(12)：1736-1740,5.DOI:10.3969/j.issn.1001-1978.2011.12.025
三羟基异黄酮对APP695基因转染PC12细胞凋亡的保护作用
Protective effect of genistein on the apoptosis of PC12 cells transfected with mutant APP695 type
摘要
Abstract
Aim To investigate the protective effect ofgenistein against apoptosis of PC 12 cells induced by tra-nsfection mutant APP695 plasmid DNA and its mechanisms. Methods PC 12 cells were transfected with pIRES2-EGFP plasmid or pIRES2-EGFP/APP695MTexpression plasmid, and then were divided into control group, vector transfected group, APP695 transfected group and GST treatment group. Flow cytometry was applied to detect cell apoptosis. Caspase-3 protein expression in PC 12 cells was obseved by Immunocyto-chemistry SABC and Western blot. Results Compared with vector transfected group, PC 12 cells in APP695 transfected group,apoptotic rate was increased significantly ( P < 0.01 ), and the expression ofCaspase-3 protein was strongly increased( P < 0. 01 ). Compared with APP695 transfected group, PC 12 cells in GST treatment group, apoptotic rate was decreased significantly ( P < 0. 01 ), and the expression of Caspase-3 protein was significantly decreased( P < 0. 01 ). Conclusion GST can reduce apoptotic rate in PC 12 cells induced by transfection mutant APP695 plasmid DNA, which might be correlated with the expression reduction of Caspase-3 protein.关键词
三羟基异黄酮/APP695/PC12细胞/凋亡/Caspase-3/保护作用Key words
genistein/ APP695/ PC 12 cell/ apoptosis/ Caspase-3 / protective effect分类
医药卫生引用本文复制引用
王凤斌,龙云,王亚南,丁振禹,赵翠香..三羟基异黄酮对APP695基因转染PC12细胞凋亡的保护作用[J].中国药理学通报,2011,27(12):1736-1740,5.基金项目
山东省自然科学基金资助项目(No Y2004C29) （No Y2004C29）
山东省科技发展计划项目(No 2007GG30002007) （No 2007GG30002007）
