解放军医学杂志2011，Vol.36Issue(9)：959-961,3.
HBV(ayw)转基因小鼠HBV复制中间体的检测
Detection of HBV replicated intermediates in the liver of HBV (ayw) transgenic mice
摘要
Abstract
Objective To establish highly sensitive and stable chemiluminescent Southern blotting system, and to detect the replication levels of HBV DNA in liver, kidney, spleen and muscles of HBV (ayw) transgenic mice. Methods The HBV probe was labeled with digoxigenin with PCR, and the sensitivity and reproducibility of hybridization system were detected by pTHBV1047. The total genomic DNA was extracted from the tissues of HBV transgenic mice and digested with Hind IE for 18 hours. After eletrophoresis, the digested DNA was transferred to nylon membrane. The replicated intermediates in the liver and other tissues of HBV transgenic mice were detected by chemiluminescent hybridization Southern blotting system. Results The sensitivity of the detection 9ystem labeled with digoxigenin was about 0. Lpg, and the hybridization system was stable and reliable. Both the integrated transgene of HBV DNA and the HBV replicated intermediates were detected in the liver of HBV transgenic mice, but the level of HBV replication was lower than that in HepG2. 2.15 cell line. HBV DNA replication also existed in kidney, spleen and muscles, but the replicated levels in these tissues were lower than that in the liver. Conclusions The 27th generation of HBV (ayw) transgenic mice is replicated in HBV transgenic mice with HBV gene stably integrated in the genome thereof.关键词
小鼠,转基因/肝炎病毒,乙型/双杂交系统技术Key words
mice, transgenic/ Hepatitis B virusi two-hybrid system techniques分类
医药卫生引用本文复制引用
陈阳述,尤玉琴,苏蔚,易学瑞,袁有成,陈文吟,张锋,孔祥平..HBV(ayw)转基因小鼠HBV复制中间体的检测[J].解放军医学杂志,2011,36(9):959-961,3.基金项目
“艾滋病和病毒性肝炎等重大传染病防治”科技重大专项“十一五”课题(2008ZX10002-011) （2008ZX10002-011）
