中南大学学报（自然科学版）2011，Vol.42Issue(7)：1890-1898,9.
细柱五加叶中抗HMGB1总三萜活性物质的闪式提取工艺
Smashing tissue extraction of active triterpenoids of anti-HMGB1 from leaves of Acanthopanax gracilistylus W.W.Smith
摘要
Abstract
Smashing tissue extraction was used to distill the active triterpenoids of anti-HMGBl from AGS, and the quadratic general rotary unitized design was used to optimize the extraction process. Taking the overall score as the target, the influences of ethyl alcohol concentration, the extraction time and the solvent amount on extraction process were studied. Finally, the optimal extraction technological conditions were determined. The triterpenoids in extraction was determined by UV. The results show that the optimal condition is taking 53% ethyl alcohol as the solvent and distilling for 175 s. This craft is stable and the reproduction quality is good (the relative standard deviation (RSD) is 0.77%), also the operation is simple and takes shorter time. Using acankoreanogenin A as comparison, saturation NaHCO3 as solvent and the most suitable wavelength is 218 nm, the liner range is 0.01-0.07 g/L (R=0.999 6) and the recovery of acankoreanogenin A is 98.2% (RSD is 1.23%).关键词
细柱五加/五加皂苷/二次通用旋转实验设计/三萜成分/闪式提取法/紫外光谱Key words
Acanthopanax gracilistylus W.W. Smith/acankoreoside A/quadratic general rotary unitized design/triterpenoids/smashing tissue extraction/ultraviolet分类
医药卫生引用本文复制引用
刘向前,郑礼胜,翁柳娜,邹方卉,李丽丽..细柱五加叶中抗HMGB1总三萜活性物质的闪式提取工艺[J].中南大学学报（自然科学版）,2011,42(7):1890-1898,9.基金项目
教育部留学回国人员科研启动基金资助项目(教外司留[2005]55号):湖南中医药大学科研启动基金(48010101-7) （教外司留[2005]55号）
中南大学研究生创新项目(2009ssxt046) （2009ssxt046）
