中国药房2011，Vol.22Issue(25)：2322-2324,3.
细菌性感染模型大鼠腹腔渗出液的上清液抗真菌作用研究
Fungicidal Effect of Peritoneal Exudates Supernatant in Bacterial Infection Rats
摘要
Abstract
OBJECTIVE: To investigate the fungicidal activity of the substance in peritoneal exudates supernatants in bacterial infection rats, and to provide reference for developing new natural fungicidal drugs. METHODS: Rats were divided into normal control group and model group with 10 rats in each group. Model group was injected with Staphyloccocus aureus intraperitoneally (3.6×l08 cfu·mL-1) to establish bacterial infection model, and model rats were sacrificed 24 h after modeling to collect, separate and treat peritoneal exudates. The fungicidal activity of peritoneal exudation, cellular fluid, supernatant on Candida albicans was observed, and the fungicidal activity of supernatant on typical fungus and effect of temperature on fungicidal activity were observed. The duration of fungicidal activity was observed under microscope. RESULTS: Compared with normal control group, large amount of peritoneal exudates emerged in model group 24 h after modeling. There were a great of white cell in peritoneal exudates and protein (P<0.01) in supernatants which possessed same fungicidal activity as cellular fluid. The peritoneal exudation supernatant possessed the most potent fungicidal activity on C. Albicans, Oidium tropioale and Candida glabrata. C. Albicans morphology changed gradually and then was expired due to disruption lastly after treatment with the supernatant, and the fungicidal activity of peritoneal exudates supernatants was destroyed dramatically after incubated at 56 t. CONCLUSION: The supernatant of peritoneal exudates from bacterial infection rats possesses potent fungicidal activity by influencing cell wall/cytomembrane of fungus. And there may be unconcerned and undiscovered antibacterial protein/peptide.关键词
金黄色葡萄球菌/细菌性感染/大鼠/腹腔渗出液/上清液/抗真菌作用Key words
Staphyloccocus aureus/Bacterial infection/Rats/Peritoneal exudates/Supernatant/Fungicidal effect分类
医药卫生引用本文复制引用
韦桂宁,何德明,宋大彦,李艳,梁宁生..细菌性感染模型大鼠腹腔渗出液的上清液抗真菌作用研究[J].中国药房,2011,22(25):2322-2324,3.基金项目
广西科学基金项目(桂科自0832231) （桂科自0832231）
广西医疗卫生重点科研课题(重200707) （重200707）
