中国药房2011，Vol.22Issue(25)：2325-2327,3.
L-氨基酸氧化酶对人肺腺癌A549细胞增殖、凋亡的影响研究
Effects of LAAO on Proliferation and Apoptosis of Human Lung Cancer Cell A549
摘要
Abstract
OBJECTIVE: To investigate the effects of L-amino acid oxidas (LAAO) on the proliferation and apoptosis of human lung cancer cell A549, and to provide a new therapeutic option for lung cancer. METHODS: Cell A549 suspension (5×107 mL) was prepared, then divided in to 4 groups according to the concentration of LAAO: 2.41,4.82, 9.64 and 19.28 mg·mL-1 intervention groups. All the groups were treated for 24, 48 and 72 h. Cellular morphology changes were observed under microscope. The proliferation inhibitory rate was evaluated by MTT assay. The cell apoptosis index was determined by TUNEL method, and compared with negative control group. RESULTS: LAAO could inhibit the proliferation of A549 cells in a dose-time-dependent manner (P<0.05). With the increase of LAAO concentration and the duration of administration, the cell apoptosis index was significantly increased (P<0.05). CONCLUSION: LAAO can inhibit the proliferation and induce apoptosis of A549 cells in dose-time-dependent manner.关键词
L-氨基酸氧化酶/肺腺癌/A549细胞/增殖/凋亡Key words
L-amino acid oxidas/Lung cancer/A549 cell/Proliferation/Apoptosis分类
医药卫生引用本文复制引用
张小润,张程,王晓宇,张湘燕,李敏,叶贤伟,夏婧..L-氨基酸氧化酶对人肺腺癌A549细胞增殖、凋亡的影响研究[J].中国药房,2011,22(25):2325-2327,3.基金项目
贵州省科学技术基金(黔科J字20052051) （黔科J字20052051）
