感染、炎症、修复2011，Vol.12Issue(3)：144-148,5.
内毒素耐受和拟胆碱药物对巨噬细胞分泌肿瘤坏死因子-α的影响
Effects of endotoxin tolerance;and;cholinergic drug on macrophages in secreting tumor necrosis factor-α
摘要
Abstract
Objective:To investigate the difference in regulating tumor necrosis factor-α (TNF-α) secretion between endotoxin tolerance and cholinergic drug-carbachol pre-treated macrophages. Methods: ①The peritoneal macrophages were harvested from male mice, and they were exposed to endotoxin (LPS) for 0. 2, 4, and 8 hours. ②The isolated peritoneal macrophages of mice were divided into 3 groups: control group (RPMI1640 routine culture), LPS pre-treatment group (pre-exposure to LPS followed by LPS stimulation), and LPS stimulation group. ③The peritoneal macrophages of rats were isolated and were divided into 3 groups: control, LPS stimulation, and carbachol pre-treatment (LPS stimulation following pre-exposure to carbachol) groups. Lysate of macrophages and cell supernatants in all experiments were harvested to determine the content of TNF-α by enzymelinked immunoadsorbent assay (ELISA) method. Results: The amount of TNF-α secretion in LPS pre-treated macrophages was not increased following being stimulated by LPS again, and no difference was observed between LPS pre-treated and control groups. No increase in TNF-a content was observed in LPS pre-treated macrophages. But for the LPS untreated macrophages, the amount of secreted TNF-a was increased dramatically accompanying with an increase in TNF-a content in the macrophages after being stimulated by LPS, showing that the quantity of both secreted and reserved TNF-a was significantly high. For carbachol pre-treated cells, the amount of TNF-α secretion was lower than in that of carbachol untreated cells, but the content of TNF-a in cells was higher than that in the control (P＜0. 01), and it was close to that in the LPS stimulating cells. Conclusions After being stimulated by LPS, the secretion of TNF-a from both endotoxin tolerant and cholinergic pre-treated macrophages is reduced, but there is a discordance in TNF-a content in the two groups. It is suggested that in macrophages, the mechanisms of inflammatory change induced by endotoxin tolerance and cholinergic anti-inflammation are different.关键词
巨噬细胞/内毒素耐受/拟胆碱药/肿瘤坏死因子-αKey words
Macrophage/ Endotoxin tolerance/Cholinergic/Tumor necrosis factor -α引用本文复制引用
吕艺,周国勇,胡森..内毒素耐受和拟胆碱药物对巨噬细胞分泌肿瘤坏死因子-α的影响[J].感染、炎症、修复,2011,12(3):144-148,5.基金项目
国家自然科学基金面上项目(30770844) （30770844）
