发光学报2011，Vol.32Issue(6)：628-635,8.DOI:10.3788/fgxb20113206.0628
头孢噻肟钠和氯霉素与牛血清白蛋白相互作用的荧光光谱分析
Fluorescence Analysis of The Interaction Between Cefotaxime Sodium and Chloramphenicol as Well as Bovine Serum Albumin
摘要
Abstract
In Tris-HCl buffer medium of Ph 7. 40, the binding reaction between eefotaxime sodium ( CTX) and chloramphenicol (CHL) with bovine serum albumin (BSA) at different temperatures was studied by fluorescence spectroscopy. The results show that the values of binding constants decrease with the increasing temperature, the quenching mechanism of the combination for BSA and drugs is a static procedure. In addition, the competitive experiments suggest that the primary binding site for both CTX and CHL are located at site I in sub-domain II A of BSA. The values of Hill's coefficients are approximately equal to 1 in the binary systems, which indicate no cooperativeness in the drug's binding with BSA. The fluorescence would quench to alarger degree when CTX (or CHL) is added to the system of BSA-CHL (or BSA-CTX). Binding constants of one drug with BSA decrease when the other drug is added. Hill's coefficients of the ternary systems are less than 1. It showed that the negative cooperativity is found when CTX and CHL bound to BSA simultaneously. Thermodynamic parameters indicate that the electrostatic interaction playes a major role in the binding of drugs with BSA.关键词
荧光光谱法；头孢噻肟钠；氯霉素；牛血清白蛋白Key words
fluorescence spectroscopy/ cefotaxime sodium/ chloramphenicol/ bovine serum albumin分类
化学化工引用本文复制引用
刘保生,王晶,薛春丽,杨超,吕运开..头孢噻肟钠和氯霉素与牛血清白蛋白相互作用的荧光光谱分析[J].发光学报,2011,32(6):628-635,8.基金项目
国家自然科学基金(20675024)；河北省重点基础研究项目(10967126D)资助项目. （20675024）
