浙江农林大学学报2011，Vol.28Issue(4)：653-661,9.
旱柳根系蛋白质双向电泳体系的建立
A two-dimensional electrophoresis system of Salix mats udana root proteomics
摘要
Abstract
To establish a suitable two-dimensional electrophoresis technology, for bettering the use of proteomic technology in discovering new proteins of Salix matsudana roots induced by salt-stress and in revealing differences of protein expression of the roots with fresh water and salt-stress, this study adopted an improved phenol extraction method to extract total proteins from S. Matsudana roots. Results were compared for different treatment conditions, and then two-dimensional electrophoresis maps of roots with treatments of 1/4 stregth Hoagland hydroponic medium and 1/4 stregth Hoagland hydroponic medium containing 100 mmol·L-1 NaCl, were compared after 24 h of salt-stress. Results showed that the improved phenol extraction method could not only extract root proteins enough for 2-D, but it could also effectively remove salt from samples. Results showed that 900 μg per immobilized pH gradient (IPG) gel, 10 min equilibrium time, and 100 000 V·h isoelectric focusing (IEF) time were the best conditions combination for the protein isolation. With the two-dimensional electrophoresis maps, 39 proteins were differentially expressed, of which 15 were down-regulated , and 24 were up-regulated with 11 newly induced.关键词
林木育种学/旱柳/蛋白质组学/双向电泳Key words
forest tree breeding/ Salix matsudana/ proteomics/salt-stress/ two-dimensional electrophoresis (2-DE)分类
农业科技引用本文复制引用
张小果,乔桂荣,李翠云,刘明英,蒋晶,李海营,邱文敏,卓仁英..旱柳根系蛋白质双向电泳体系的建立[J].浙江农林大学学报,2011,28(4):653-661,9.基金项目
国家自然科学基金资助项目(30972340) （30972340）
浙江省自然科学基金杰出青年团队资助项目(R3090070) （R3090070）
中央级非盈利性科研院所基金资助项目(RISF6920) （RISF6920）
