解放军医学杂志2011，Vol.36Issue(10)：1031-1036,6.
氩氦刀冷冻消融治疗乙型肝炎病毒相关性肝细胞癌前后PD-1的变化及其与预后的关系
Changes in PD-1 and effect thereof on prognosis of patient after argon-helium cryoablation for hepatitis B relatedhepatocellular carcinoma
摘要
Abstract
Objective To investigate the relationship of the expression of circulating programmed death-1 (PD-1) to the disease course of hepatocellular carcinoma (HCC), to explore the influence of cryoablation on the changes in PD-1 in peripheral blood, and to analyze the correlation between PD-1 and the prognosis of HCC patients. Methods The expression of circulating PD-1 was determined by flow cytometry to analyze the correlation between the expressions of PD-1 and progression of HCC. Of 141 patients with HCC, 109 underwent argon-helium cryoablation, the expressions of PD-1 in peripheral blood pre- and post-therapy were then detected and the changes were recorded thereafter. According to median percentage of PD-1 pre-cryoablation> 46 patients with early-stage HCC were assigned into tow-expression group (PD-1 4.05% ±1.28%, n=23) and high-expression group (9.26% ± 3.09%, n=23), and 63 patients with intermediate-stage HCC into low-expression group (9.26%±1. 63%, n=31) and high-expression group (18.05%±3. 78%, n=32). The tumor free survival (TFS) and overall survival (OS) were calculated and compared by using Kaplan-Meier Survival Analysis. Results The expression of circulating PD-1 were 6.66% ± 3. 52% in BCLC early-stage patients with HCC, 13. 72% ± 5.29% in intermediate-stage cases, and 20. 28%±11.89%% in advanced-stage cases. After cryoablation, the frequency of PD-1 expression decreased compared with that of pre-cryoablation. In 11 patients with tumor recurrence, the frequency of PD-1 expression rose prominently compared to that of post-cryoablatioa In both early- and intermediate-stage HCC patients, TFS and OS were shorter in patients with high expression of PD-1 compared with those with low expression of PD-1. Conclusions The expressions of circulating PD-1 increases with tumor progression. After argon-helium cryoablation, a reduction of PD-1 expression in HCC patients can be achieved. Increased expression of PD-1 may provide a reference for prediction of survival time in HCC patients after cryoablation.关键词
程序性死亡受体-1/癌,肝细胞/冷冻外科手术/预后Key words
programmed death-1/ carcinoma, hepatocellular/ cryosurgery/ prognosis分类
医药卫生引用本文复制引用
周霖,王春平,王宏,楼敏,王福生,吕吉云,杨永平,曾珍,石峰,张敏娜,陈艳,常秀娟,陆荫英,白文林,曲建慧..氩氦刀冷冻消融治疗乙型肝炎病毒相关性肝细胞癌前后PD-1的变化及其与预后的关系[J].解放军医学杂志,2011,36(10):1031-1036,6.基金项目
国家科技重大专项课题(2008ZX10002-018) （2008ZX10002-018）
首都医学发展基金课题(2007-1021,2009-2041) （2007-1021,2009-2041）
