生态学杂志2011,Vol.30Issue(10):2173-2178,6.
外源-氧化氮供体硝普钠对黑麦草幼苗耐碱性的影响
Effects of exogenous nitric oxide donor sodium nitroprusside on alkali resistance of Lolium perenne seedlings
摘要
Abstract
A sand culture experiment was conducted to study the effects of nitric oxide (NO) donor sodium nitroprusside (SNP) on the growth, leaf active oxygen metabolism, and leaf osmotica accumulation of ryegrass (Lolium perenne L.) seedlings under NaHCO3 stress. Under the stress of 100 mmol NaHCO3·L-1, applying 60μmol·L-1 of SNP could alleviate the seedling' s growth inhibition, increase the leaf O2 generation rate and H2 O2 and malondialdehyde contents, promote the leaf superoxide dismutase, guaiacol peroxidase, ascorbate peroxidase, and plasma membrane H+-ATPase activities, increase the leaf glutathione, soluble sugar, soluble protein, and proline contents and K+/Na+ ratio, and decrease the leaf catalase activity and ascorbic acid content. Little effects were observed on the leaf free amino content. These results suggested that SNP could alleviate the damage of alkali stress on Lolium perenne seedling leaves via activating the an-tioxidant system activity, promoting the osmotica accumulation, and improving the Na+ and K+ balance, and accordingly, promote the alkali resistance of the ryegrass plant.关键词
硝普钠/黑麦草/NaHCO3胁迫/活性氧代谢/渗透溶质Key words
sodium nitroprusside (SNP)/Lolium perenne L./NaHCO3 stress/active oxygen metabolism/osmotica分类
农业科技引用本文复制引用
刘建新,王鑫,王瑞娟,贾海燕..外源-氧化氮供体硝普钠对黑麦草幼苗耐碱性的影响[J].生态学杂志,2011,30(10):2173-2178,6.基金项目
甘肃省庆阳市科技支撑计划项目(GK098-1-34)资助. (GK098-1-34)