生态学杂志2011，Vol.30Issue(10)：2214,1.
稗草潜在化感物质的排查
Potential allelochemicals in barnyard grass.
摘要
Abstract
A bioassay was conducted to examine the allelopathic activities of five reported allelo-pathic substances in barnyard grass, I.e. , 2,4-bis (1,1-dimethylethyl) phenol, p-hydroxyman-delic acid, and three kinds of phthalate esters, on rice, barnyard grass, and lettuce. HPLC was used to analyze the contents of the five compounds and 2,4-dihydroxy-7-methoxy-l ,4-benzoxazin-3-one (DIM BO A) in barnyard grass tissues, root exudates, and rhizosphere soil. At concentration 100μg·ml-1, 2,4-bis (1,1-dimethylethyl) phenol had the strongest inhibitory effect on rice, lettuce and barnyard grass, while p-hydroxymandelic acid had the weakest one. Dimethyl-ester-phthalic acid, diisooctyl ester-phthalic acid, and 2, 4-bis (1,1 -dimethylethyl) phenol exhibited greater inhibition on barnyard grass than on rice. HPLC analysis showed that the p-hydroxymandelic acid concentration in barnyard grass shoots, roots, and root exudates at seedling stage was 9.72μg·g-1,7.29μg·g-1, and 0.24μg·ml-1, respectively. No p-hydroxy-mandelic acid was detected at other growth stages of barnyard grass and its rhizosphere soil. 2,4-bis (1 ,1-dimethylethyl) phenol, DIM BO A, and phthalate esters were not detected in all samples. The dose-response trial on p-hydroxymandelic acid showed no significant effect on target plants. It was concluded that p-hydroxymandelic acid was not the main allelochemicals in barnyard grass, and the reported 2,4-bis (1,1-dimethylethyl) phenol, DIMBOA, and three phthalate esters were not detected in barnyard grass.关键词
稗草/对羟基扁桃酸/2,4-二叔丁基酚/丁布/酞酸酯Key words
Echinochloa crus-galli L./p-hydroxymandelic acid/2,4-bis (1,1-dimethylethyl)phenol/DIMBOA/phthalate esters分类
农业科技引用本文复制引用
秦波,张晓影,王朋..稗草潜在化感物质的排查[J].生态学杂志,2011,30(10):2214,1.基金项目
国家自然科学基金项目(40971159和31070390)和辽宁省博士科研启动基金项目(20101123)资助. （40971159和31070390）
