华南农业大学学报2011，Vol.32Issue(3)：18-21,4.
空间诱变水稻DH104的稻瘟病抗性分析及抗病基因定位
Analysis of Resistance to Blast and Mapping of Rice Blast Resistance Gene in DH104
摘要
Abstract
The DH104, an indica rice mutant from Huhan B, and eight monogenic lines were subjected to resistance spectrum with 39 isolates collected from Guangdong Province, China. The results showed that DH104 conferred broader resistance spectrum than the eight monogenic lines. To identify and map the blast resistance gene in DH104, a representative isolate GD0193 was used to identify and map R gene from DH104. Isolate GD0193 was used for inoculating a population of F2 derived from the cross between DH104 and highly susceptible variety L101 (indica). The results showed that the segregation of resistant , and susceptible(S) progenies fitted a 3:1 ratio in the F2 population(313R: 97S, x 2 =0. 896 1), indicating the R gene was controlled by a single dominant gene, temporarily designed as Pixt(t) gene. The R gene was located between RM136 and RM7213 on chromosome 6 using SSR markers, with genetic distances 4. 9 and 1.5 cM respectively.关键词
水稻/稻瘟病/抗性分析/基因定位Key words
Oryza sativa/ rice blast/ resistance analysis/ gene mapping分类
农业科技引用本文复制引用
孙大元,肖武名,杨祁云,王慧,郭涛,刘永柱,张建国,朱小源,陈志强..空间诱变水稻DH104的稻瘟病抗性分析及抗病基因定位[J].华南农业大学学报,2011,32(3):18-21,4.基金项目
863计划项目(2007AA100101) （2007AA100101）
国家科技支撑计划项目(2008BAD97B02) （2008BAD97B02）
广东省自然科学基金(815106400100002) （815106400100002）
国家和广东省现代农业产业技术体系资助 （）
