华南农业大学学报2011，Vol.32Issue(3)：63-67,5.
SRAP体系优化及在胡枝子亲缘关系中的应用
Optimization of SRAP System and Its Preliminary Applicationin Genetic Relationship Analysis of Lespedeza
摘要
Abstract
Orthogonal design was used to optimize SRAP-PCR amplification system for Lespedeza in terms of five factors ( Taq DNA polymerase, Mg2+ , DNA template, dNTPs and primer) from four levels. An optimal SRAP-PCR system for Lespedeza was obtained as 40 ng DNA template, 1. 5 mmol/L Mg2+ , 1.0 U Taq DNA polymerase, 0. 16 mmol/L dNTPs, 0. 64 μmol/L primer for 25 μK reaction system. The phylogenetic relationship of 10 species of Lespedeza was investigated using SRAP technique. SRAP analysis produced 274 bands by using the 10 most informative primer pairs, 244(89. 01%) of which were polymorphic. Cluster analysis indicated that the 10 species of Lespedeza can be divided into four groups.关键词
胡枝子/SRAP/优化/亲缘关系Key words
Lespedeza/ SRAP/ optimization/ genetic relationship分类
农业科技引用本文复制引用
骈瑞琪,倪慧群,欧阳昆唏,李伟,陈晓阳..SRAP体系优化及在胡枝子亲缘关系中的应用[J].华南农业大学学报,2011,32(3):63-67,5.基金项目
“863”国家高技术研究发展计划(2002AA241111) （2002AA241111）
木本豆科饲料植物胡枝子产业化示范项目(2006D90204004) （2006D90204004）
