华南农业大学学报2011，Vol.32Issue(3)：68-72,5.
应用可视芯片技术检测食品中常见产毒微生物的方法研究
Detection and Identification of Foodborne Toxigenic Microbial by Using Thin-Film Biosensor Chips
摘要
Abstract
In order to develop a rapid and accurate assay, based on the thin-film biosensor chips for the detection and identification of Escherichia. Coli O157: H7, Shigella, Salmonella, Listeria monocytogenes in food. rfbE (lipopolysaccharide O157 of S. E. coli) ,invA( invasion protein of S. Salmonella) , ipaH( invasion-associated plasmid antigen H of S. Shigella) and PrfA (PrfA protein of S. L monocytogenes) were used as target genes to design primers and probes. Thus,all the olignucleotide probes were labeled with 5' polyT group whereas the reverse primers were labeled with biotin. The genes were amplified and hybridized with probes on chip surface. Under the same condition,the detected "targe"pathogens were E. coli O157: H7, Shigella, Salmonella, L. monocytogene. Detection sensitivity of this system was upto 670 cfu/mL for Shigella. This method can be used rapidly and accurately to detect four species foodborne toxigenic mi-crobial.关键词
可视芯片/产毒微生物/检测Key words
thin-film biosensor chips/ toxigenic microbial/ identification分类
生物科学引用本文复制引用
朱许强,冯家望,王小玉,邝筱珊,胡松楠,丁焕中..应用可视芯片技术检测食品中常见产毒微生物的方法研究[J].华南农业大学学报,2011,32(3):68-72,5.基金项目
国家质检总局科技计划项目(20101K171) （20101K171）
