核农学报2011，Vol.25Issue(3)：482-487,6.
响应面法优化白腐菌Pleurotus eryngii-Co007产木质素降解酶条件
OPTIMIZATION OF CONDITIONS FOR LIGNINOLYTIC ENZYMES PRODUCTION FROM WHITE ROT FUNGI Pleurotus eryngii-Co007 BY RESPONSE SURFACE METHODOLOGY
摘要
Abstract
In order to enhance the ligninolytic enzymes activity of Pleurotus eryngii-Co007 the influence of initial pH, straw, Cu2+ concentration, Tween-80 content on ligninolytic enzymes production were studied. Designed with single factor experiment and the Central Composite in the response surface methodology, the optimization conditions of producing ligninolytic enzymes were initial pH at 5. 63, 1. 12% of straw, Cu 2+ at 4. 00mmol/L, Tween-80 0. 83g/L. Under these conditions, the ligninolytic enzymes activity in fermentation broth of shake flask on the eighth day was 241. 40U/ml,which was similar to that predicted from the regression model with a relative error of 2. 18% .关键词
刺芹侧耳/木质素降解酶/产酶条件/响应面Key words
Pleurotus eryngii/ ligninolytic enzymes/ fermentation conditions/ response surface引用本文复制引用
陈敏,郭倩,姚善泾..响应面法优化白腐菌Pleurotus eryngii-Co007产木质素降解酶条件[J].核农学报,2011,25(3):482-487,6.基金项目
浙江省自然基金(Y5053340) （Y5053340）
