核农学报2011,Vol.25Issue(3):588-593,581,7.
酸雨对木芙蓉幼苗光合作用及抗氧化酶活性的影响
EFFECTS OF ACID RAIN ON PHOTOSYNTHESIS AND ANTIOXIDANT ENZYME ACTIVITY OF H.mitabilis L.SEEDLINGS
摘要
Abstract
A pot experiment was conducted to study the effects of simulated acid rain on foliar damage, chlorophyll (Chi) content, membrane permeability (MP) , antioxidant enzyme activity and gas exchange parameters in leaves of 2 - year H. Mitabilis seedlings. Four gradients of simulated acid rain solutions of pH 5. 6 ( CK) , 4. 0, 3. 0 and 2. 0 were used. Results showed that the leaves treated by pH 2. 0 and 3. 0 showed visible injury spots to various extent. The membrane permeability ( MP) increased significantly after acid rain stress with an extremely significant negative correlation to pH value ( r = - 0. 961 * * ). The maximum catalase ( CAT) activity and peroxidase ( POD) activity were observed at pH 2.0(48.75mg · Min-1 · G-1 · FW and 77. 69U · Min-1 · G-1 · FW, respectively), while that ofsuperoxide dismutase (SOD) activity were observed at the pH 3. 0(216. 80U · G-1 · FW). Chlorophyll a (Chi a), chlorophyll b ( Chi b) , total chlorophyll content, Chi a/b, net photosynthetic rate ( Pn) , stomatal conductivity ( Gs) and transpiration rate ( Tr) decreased with decreaseing pH value of pH, and intercellular CO2concentration ( Ci) showed a trend of decreasing first and then increasing, while water use efficiency ( WUE) was opposite. The visible damage threshold of H. Mitabilis by simulated acid rain was less than pH 2. 0, and the threshold on the physiological activity was among pH 2.0 to 3.0, which indicated that H. Mitabilis had higher tolerance to acid rain stress. This study could provide theoretical reference for cultivation management and resistance screening of H. Mitabilis, and offer basic theory to choose the greening tree species in Southern cities.关键词
木芙蓉/模拟酸雨/光合作用/抗氧化酶活性Key words
H.mitabilis L. / simulated acid rain/ photosynthesis/ antioxidant enzyme activity引用本文复制引用
王应军,邓仕槐,姜静,尚鹤,林波,孙亚琴,胡晓梅..酸雨对木芙蓉幼苗光合作用及抗氧化酶活性的影响[J].核农学报,2011,25(3):588-593,581,7.基金项目
国家"十一五"计划攻关项目(2006BAD03A0104-2),四川省教育厅重点项目(10ZA059) (2006BAD03A0104-2)