基础医学与临床2011，Vol.31Issue(10)：1088-1093,6.
黄芪注射液对低氧低糖/复氧复糖大鼠海马神经元JNK3表达的影响
Effect of Astragalus injection on the expression of JNK3 after hypoxia/hypoglycemia and reoxygenation in rats hippocampal neurons
摘要
Abstract
Objective Investigate the effect of Astragalus injection on the expression of JNK3 ( C-JUN N terminal kinase) following hypoxia/ hypoglycemia and reoxygenation in rat hippocampal neurons. Methods 8 days-old hip-pocampal neurons which had undergone primary cultures were divided randomly into normal control group Nhypoxia/ hypoglycemia and reoxygenation group (model group) , astragalus injection group and vehicle control group. Hippocampal neurons were treated with reoxygenation after deprived of oxygen and glucose for 30 minutes in model group, and expressions of JNK3 were measured by immunohistochemistry and western blotting after reoxygenation 0,0. 5,2,6,24,72,120 h . Results Compared with normal control group, the numbers of JNK3-marked neurons and the mean optic density (MOD) of JNK3 protein increased obviously in model group excepted 120 h (29. 3 ± 1. 3 vs 3. 1 ±0. 8 ,P <0. 05 ;0. 765 ±0.06 vs 0.448 ±0. 06,P <0. 05); Compared with model group, the numbers of JNK3-marked neurons and MOD of JNK3 decreased obviously in the Astragalus injection group excepted 120 h (18. 2 ±1.5 vs 29.3 ±1.3,P <0.05;0.658 ±0.04 vs 0.765 ±0.06,P <0.05). Conclusions Astragalus injection inhibits the expression of JNK 3 protein following hypoxia / hypoglycemia and reoxygenation , which plays a role in inhibiting hippocampal neuronal apoptosis.关键词
缺氧缺糖/复氧复糖/黄芪注射液/C-JUN氨基末端激酶/海马神经元Key words
hypoxia/hypoglycemia and reoxygenation/ Astragalus injection/ C-JUN N terminal kinase 3/ hippocampal neuron分类
医药卫生引用本文复制引用
叶冬青,钱涛,高维娟,闫凤霞,张雅丽,侯志平..黄芪注射液对低氧低糖/复氧复糖大鼠海马神经元JNK3表达的影响[J].基础医学与临床,2011,31(10):1088-1093,6.基金项目
国家自然科学基金项目(81072923) （81072923）
