江西农业学报2011，Vol.23Issue(6)：1-7,22,8.
水稻种质资源指纹与多样性分析的分子标记体系研究
Molecular Marker Systems for Fingerprinting and Diversity Analysis of Rice Germplasm Resources
摘要
Abstract
The genetic variability and relationships among 265 rice germplasm accessions from the Genebank of Philippine Rice Research Institute were established from DNA fingerprints generated by two resistance gene analogue (RGA) markers; C - terminal leucine-rich repeat (CLRR) and ribonuclease inhibitor leucine -rich repeat (RLRR), and two universal primer markers; Seoulin Research Institute Life Science - 6 ( SRILS6) and Seoulin Research Institute life Science - 8 (SRILS8 ). The DNA fingerprints showed that CLRR was the most discriminating marker, generating 48 polymorphic bands in the germplasm samples with an average of 28.6 bands per accession, thus it was the most efficient marker for rapid DNA fingerprinting analysis. On the average, the RGA and SRILS primer markers showed the similar power in detecting the polymorphism within the population at the entire germplasm population level, and the average percentage of polymorphic bands generated by RGA and SRILS primer markers was 60.6% and 60. 5% , respectively. Seven dendrograms were generated based on the banding profiles generated by RGA and SRILS primers or their combinations , featuring two major groups: group I and group Ⅱ. Dendrogram analysis showed that both RGA and SRILS primers could distinguish all 265 accessions, and revealed a high level of diversity among the accessions. The clustering patterns and the compositions of the groups had the similarity of 88% ～ 97% among the seven dendrograms. This indicated that the genomic regions amplified by these particular RGA and SRILS markers might have undergone mutations at comparative rate so as to manifest highly consistent genetic trees.关键词
水稻；种质资源；DNA指纹图谱；RGA；SRILS；聚类分析Key words
Rice/Germplasm resources/DNA fingerprinting/RGA/SRILS/Clustering analysis分类
农业科技引用本文复制引用
万勇,刘红梅,胡标林,李霞,Gabriel O.ROMERO,谢建坤..水稻种质资源指纹与多样性分析的分子标记体系研究[J].江西农业学报,2011,23(6):1-7,22,8.基金项目
现代农业产业技术体系建设专项(NXCYTY-01)；江西省重大招标项目“东乡野生稻有利基因的发掘、定位及标记辅助选择育种研究”. （NXCYTY-01）
