江西农业学报2011，Vol.23Issue(6)：30-33,4.
一株高效抑制烟草青枯病菌的烟田土壤细菌的分离与鉴定
Isolation and Identification of A Soil Bacterial Strain Efficiently Inhibiting Ralstonia solanacearum in Tobacco Field
摘要
Abstract
A bacterial strain, which was isolated from the healthy soil sample in Qianjiang tobacco district of Chongqing city, was found to possess obvious inhibitory action on Ralstonia solanacearum in tobacco. Flat confrontation test showed that the bacterial strain against Ralstonia solanacearum had the inhibition zone of 28.9 mm in diameter. In the further study, the extracellular products of the strain were crudely extracted and taken liveness detection. The diameter of inhibition zone of the crude extract at the concentration of 250 μg per piece was 15.5 mm tested by filter paper method. IC50 of the crude extract was 342 μg/mL by MIC method, and the inhibition ratio decreased with the extension of time, but still keeping above 70% 3 days later. According to the morphological characteristics, culture character, cellular fatty acid and 16S rDNA sequence analysis of this bacterial strain, it was identified to be Pseudomonas aeruginosa.关键词
Ralstonia solanacearum；抑菌作用；Pseudomonas aeruginosa；分离；鉴定Key words
Ralstonia solanacearum/Bacteriostasis/Pseudomonas aeruginosa/Isolation/Identification分类
农业科技引用本文复制引用
董夏伟,缪莉,靳翠丽,董昆明,周晓见..一株高效抑制烟草青枯病菌的烟田土壤细菌的分离与鉴定[J].江西农业学报,2011,23(6):30-33,4.基金项目
国家自然科学基金项目(41076097、41006097)；江苏省自然科学基金项目(BK2010322)；江苏省环境材料与环境工程重点实验室开放课题(K090027、K090025、K090026、K090028)；江苏省教育厅研究生科技创新项目(M080960)；扬州大学“新世纪”人才工程支持项目. （41076097、41006097）
