中国组织工程研究与临床康复2011，Vol.15Issue(42)：7847-7850,4.DOI:10.3969/j.issn.1673-8225.2011.42.014
硫酸庆大霉素-壳聚糖纳米粒的制备及性能评价
Preparation and characteristics of gentamicin sulfate-loaded chitosan nanospheres
李亮亮 1王黎明 1徐燕1
作者信息
- 1. 南京医科大学附属南京第一医院骨科,江苏省南京市,210001
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摘要
Abstract
BACKGROUND: Gentamicin beads as a local drug delivery system was earlier used for tie treatment of chronic osteomyelitis ofthe local drug delivery system, but due to its degradation and absorption in the body, it cannot be the second out, thus itsapplication is limited. Therefore, domestic and foreign scholars have been working to study absorbable materials loaded withantibiotics device.OBJECTIVE: To formulate the gentamicin sulfate-loaded chitosan nanospheres (GS-CS nanospheres) and to evaluate itsperformance and to study its releasing behavior and the anti-staph a u re us efficacy in vitro.METHODS: The GS-CS nanospheres were prepared by ion cross-linking technique using chitosan as drug carrier andgentamicin sulfate as model drug and sodium trip olyphosp hate as cross linker reagent. The drug loading and encapsulationefficiency were estimated. The releas ing behavior of the GS-CS nanospheres was studied and the diameter of inhibition zone ofthe GS-CS nanospheres was measured.RESULTS AND CONCLUSION: The nanospheres were s ph eric a I with the diameter ranging from 40 nmto 70 nm, theencapsulation efficiency and drug loading were estimated to be 31.3% and 15.4%, respectively, toirtn releasing behavior coufclco nti n ue a b o ut 14 days; th e a nt I-sta p h a u re us eff ica cy in vitro co u I d c o nti n u e a b o ut 25 days .the gr e atest a nti-staph a ureusefficacy reached at 5 days and then decreased gradually.关键词
硫酸庆大霉素/壳聚糖/纳米粒/体外释放/骨髓炎分类
基础医学引用本文复制引用
李亮亮,王黎明,徐燕..硫酸庆大霉素-壳聚糖纳米粒的制备及性能评价[J].中国组织工程研究与临床康复,2011,15(42):7847-7850,4.
