林业科学2011，Vol.47Issue(7)：128-132,5.
唐菖蒲2种主要病毒的多重RT-PCR检测
Detection of Two Viruses of Gladiolus hybridus by Multiplex RT-PCR
摘要
Abstract
Specific primer pairs were designed according to the gene sequence of cucumber mosaic virus( CMV ) and tobacco mosaic virus (TMV) coat protein region. The two viruses could be detected by simple RT-PCR with the primers and the suitable primers were selected for multiplex RT-PCR. Using 18S rRNA of gladiolus as an internal control, a multiplex RT-PCR program was established to simultaneously detect the two viruses of Gladiolus hybridus. Two specific bands,CMV(629 bp)and TMV(423 bp)were amplified from all the samples infected with CMV and TMV by the multiplex RT-PCR. Sequence analysis of the amplified products showed that the nucleotide sequences homology of CMV and TMV was 93% ~97% and 99% , respectively, compared with the sequences of other isolates.关键词
唐菖蒲/多重RT-PCR/黄瓜花叶病毒/烟草花叶病毒/18S rRNAKey words
Gladiolus hybridus/ Multiplex RT-PCR/ CMV/ TMV/ 18S rRNA分类
农业科技引用本文复制引用
胡小燕,陈莉,辛海波,连青龙,义鸣放..唐菖蒲2种主要病毒的多重RT-PCR检测[J].林业科学,2011,47(7):128-132,5.基金项目
国家自然科学基金项目(30471221). （30471221）
