基础医学与临床2011，Vol.31Issue(10)：1099-1103,5.
中枢组胺在心理应激致大鼠哮喘发病中的作用机制
Mechanism of central histamine in rats with asthma induced by psychological stress
摘要
Abstract
Objective This study aims to investigate the mechanism of central histamine on psychological stress rats with asthma. Methods Healthy SD rats were randomly assigned as 4 test groups and me control group. Asthma and psychological stress rat models were established. Pulmonary function was tested by Powlab biological signal processing system. Serum corticosterone ( CORT) was tested by radioimmunoassay. Serum IL-4, IFN-γ were tested by enzyme-linked immunosorbent assay, which were used to calculate the ratio of Thl/Th2. Lung tissues were stained with HE. Histamine content in the hypothalamus was determined by HPLC. And through intracerebroven-tricular injection of histamine H1 receptor antagonist,chlorpheniramine,to observe pulmonary function and Thl/Th2 ratio in rats with asthma. Results After the psychological stress, compared with the asthma model group, pulmonary function of stress asthmatic rats decreased ( P < 0. 05 ). Serum Thl/Th2 ratio were decreased ( P < 0. 05 ). Besides, peripheral inflammation of lung tissue were worse. Histamine in the hypothalamus was increased from 231 ±32 nmol/g to 287 ±44 nmol/g(P <0. 05). However, after intracerebroventricular injection of histamine H1 receptor antagonist, chlorpheniramine, pulmonary dysfunction of the stressed asthmatic rats and inflammation of lung tissue were improved. Conclusions Psychological stress can increase central histamine levels in rats with asthma, and elevated histamine acts on H1 receptors, that plays a important role in the pathogenesis of airway hyperrespons-iveness and peripheral inflammation.关键词
心理应激/哮喘大鼠/中枢组胺/组胺H1受体Key words
psychological stress/ asthmatic rate/ central histamine/ histamine H1 receptor分类
医药卫生引用本文复制引用
张红叶,宋佳贤,杨莉亚,杨八双,黄旭,董榕..中枢组胺在心理应激致大鼠哮喘发病中的作用机制[J].基础医学与临床,2011,31(10):1099-1103,5.基金项目
国家大学生创新性实验计划项目(G2007061) （G2007061）
