基础医学与临床2011，Vol.31Issue(10)：1110-1114,5.
氯喹抑制体外培养星形胶质细胞的激活
Chloroquine inhibits the activation of astrocyte in vitro
摘要
Abstract
Objective To investigate the effects of Chliroquine on Pentylenetetrazole(PTZ)- activated hippocampal astrocytes of rats. Methods eonatal rat (24h) hippocampal astrocytes were obtained and divided into control group, PTZ-activated group and Chliroquine groups with Chliroquine treatment at 25, 50, 75 mg/L. The cell proliferation , expression of glial fibrillary acidic protein ( GFAP) and CyclinDl after the treatments were detected with MTT assay, immunofluorescence cytochemistry and western blot respectively. Results N Results In comparison with control group, PTZ could induce the activation of astrocytes and increase the expressions of GFAP and CyclinDl (P<0. 05) , In comparison with PTZ-activation group, Chliroquine treatment resulted in significant inhibition of the cell proliferation, GFAP and CyclinDl in the PTZ-activated astrocytes ( P < 0. 05 ). Chliroquine at 75 mg/L showed the strongest inhibitory effects against astrocyte activation while maintained nearly normal level of astrocyte activation in comparison with the control group. Conclusions Chliroquine could inhibit PTZ-induced activation of astrocytes, suggesting that chliroquine may function as potential medicine for epilepsy.关键词
星形胶质细胞/癫痫/戊四氮/氯喹Key words
astrocyte/ epilepsy/ pentylenetetrazole/ chliroquine分类
医药卫生引用本文复制引用
荆丽丽,吴淑华..氯喹抑制体外培养星形胶质细胞的激活[J].基础医学与临床,2011,31(10):1110-1114,5.基金项目
山东省教育厅科技计划资助项目(JO8LG14) （JO8LG14）
