基础医学与临床2011，Vol.31Issue(10)：1120-1123,4.
TRAIL诱导Hela细胞凋亡的线粒体信号通路
TRAIL induced apoptosis in Hela cells by mitochondrial signal pathway
摘要
Abstract
Objective To explore the mitochondrial pathway in the apoptosis of Hela cells induced by TRAIL. Methods Apoptotic cells were detected by DNA electrophoresis. The mitochondria membrane potential (MMP), the expressions of BCL-2, the location of Cyt c and AIF, the caspase-3 activity were tested by confocal laser microscopy , western blot, immunofluorescence and caspase-3 activity assay. Results TRAIL can induce apoptosis in Hela cells. DNA ladders were showed on agarose gel electrophoresis. At the same time, TRAIL induced the decrease of MMP (P <0. 05 ,P <0. 01) and BCL-2, the release of Cyt c, the translocation of mitochondrial AIF to cytoplasm and nuclei, the increase of caspase-3 activity (the activity increased to 2. 25 ±0. 20 fold in 10 h treated cells, P < 0. 05 ) in the time-dependent manner. Conclusions TRAIL induce the apoptosis of Hela cells through involves mitochondrial signal pathway.关键词
TRAIL/Hela细胞/细胞凋亡/线粒体Key words
TRAIL/ hela cells/ apoptosis/ mitochondria分类
医药卫生引用本文复制引用
叶记林,刘永春,于有江,刘延庆..TRAIL诱导Hela细胞凋亡的线粒体信号通路[J].基础医学与临床,2011,31(10):1120-1123,4.基金项目
扬州环境资源职业技术学院基金(07001) （07001）
