中国肿瘤生物治疗杂志2011，Vol.18Issue(5)：490-495,6.DOI:10.3872/j.issn.1007-385X.2011.05.005
雌激素受体亚型对乳腺癌MCF-7细胞生长及微环境中Th平衡的影响
Effects of estrogen receptor subtype on growth of breast cancer MCF-7 cells and Th balance in microenvironment
摘要
Abstract
Objective: To study estrogen receptor ( ER) subtype on the growth of breast cancer cell line MCF-7 and the secretion of Th1 and Th2 cytokines in MCF-7 tumor microenvironment. Methods; Era or Erβ expression in MCF-7 cells was silenced by RNA interference and MCF-7 cells with different Era/Erp expression status were obtained. MTT test, flow cytometry and RT-PCR assay were used to detect proliferation, cell cycle and expression of apoptosis suppressor genes. Secretion of IFN-7 and IL-4 in cell supernatant were analyzed by ELISA assay. Results; After RNA interference, protein levels of Era or Erβ in MCF-7 cells decreased by (77. 7 ± 3. 3 )% or (68. 3 ±2. 1)% , respectively. Compared to control group, after knocking down Era gene expression, MCF-7 cells grew slower (P < 0.05) and were arrested at phase G0 ~ G1, expression of apoptosis suppressor gene XIAP decreased by (43.0±2.0)%. And the level of IFN-γ increased by (1. 89 ±0. 34) times. However, after knocking down the Erp gene expression, MCF-7 grew faster (P < 0. 05 ) , and the proportion of cells entering S phase increased, the expression of apoptosis suppressor genes Bcl-2, Bcl-xl and XIAP increased by (1.28 ± 0.21) times, (1.61 ± 0. 32) times and (1. 65 ± 0. 29 ) times, respectively, while the level of IFN-7 decreased by (28.0 ±4.0)% , compared to the control group. Conclusion; The expression status of ERsubtype can affect the growth of MCF-7 cells and induce the Th bias in microenvironment by regulating the autocrine levelof IFN-γ.关键词
雌激素受体/RNA干扰/Th平衡/乳腺癌/ERα/ERβKey words
estrogen receptor(ER)/ RNA interference/ Th balance/ breast cancer/ Era/ Erβ分类
医药卫生引用本文复制引用
牛秀珑,叶路,毛立群,王越..雌激素受体亚型对乳腺癌MCF-7细胞生长及微环境中Th平衡的影响[J].中国肿瘤生物治疗杂志,2011,18(5):490-495,6.基金项目
国家自然科学基金资助项目(No.81041071) （No.81041071）
天津市自然科学基金资助项目(No.08JCYBJC06900) （No.08JCYBJC06900）
武警医学院科学技术研究面上项目资助(No.WY200802). （No.WY200802）
