武汉工程大学学报2011，Vol.33Issue(7)：31-33,3.DOI:10.3969/j.issn.1674-2869.2011.07.009
气相色谱法分析蒙药白头翁多糖的单糖组分
Component analysis of monosaccharide in polysaccharides for Mongolian Medicine Pulsatilla chinensis by gas chromatography
摘要
Abstract
The GC analysis was used to study the monosaccharide composition of polysaccharide in Pulsatilla chinensis polysaccharide after hydrolyzing and acetylation. The monosaccharide components and the molar ratio is Mrharmnose: Marabincse: Mxylose.: Minositol: Mmannose: Mglucose= 10. 22: 2. 3410. 45:1. 28: 2. 41: 1. 82. Gas chromatography conditions are as follow: column for the Thermo TR-5ms, temperature-programmed to 100 ℃ and then 260 ℃, the injection amount of 1 μL. We used the peak area of standard samples and internal standard curve method to analyze these monosaccharides, respectively. The linear regression equation was Y = 0. 446X + 0. 057 5(r = 0. 999 0), recovery was 103.04%, and RSD was 0.39%.关键词
白头翁/气相色谱/多糖Key words
Mongolian Medicine Pulsatilla chinensis/ gas phase chromatography/ polysaccharide分类
化学化工引用本文复制引用
徐秀廷,赵玉英,顾德昌..气相色谱法分析蒙药白头翁多糖的单糖组分[J].武汉工程大学学报,2011,33(7):31-33,3.基金项目
内蒙古自然科学基金(2010MS0213) （2010MS0213）
