疑难病杂志2011，Vol.10Issue(12)：914-916,3.DOI:10.3969/j.issn.1671-6450.2011.12.016
大鼠低氧低温睡眠剥夺后血清TNF-α和IL-6水平变化的研究
Investigation on the changes of TNF-α and IL-6 in SD rats with sleep deprivation under hypoxia and hypothermia
摘要
Abstract
Objective To investigate the concentration of TNF-a and IL-6 in blood serum level in sleep deprivation model of SD rats under hypoxia and hypothermia conditions. Methods Healthy adult SD rats were randomly allocated into five groups including antitheses group ( CC group ), sleep deprivation group( SD0 ) and sleep deprivation group at the 1st day, the 4th day and the 7th day ( SD, ,SD4 ,SD7 ) under hypoxia and hypothermia conditions. ELISA were used to test the concentration of the blood serum of TNF-a and IL-6. Results ( 1 )Compared with the CC group, the concentration of blood serum TNF-a in SD, ,SD4 and SD7 group rise significantly( P <0. 05 andP <0. 01 );there was no significant different in TNF-a concentration between SD0 and SD, group( P >0.05 ), while the TNF-a concentration in SD4 and SD7 group rise significantly( P < 0.01 ). The serum TNF-a concentration tends to rise as the sleep deprivation prolongs.( 2 )Compared with the CC group, the concentration of blood serum IL-6 in SD, ,SD4 and SD7 group rise significantly( P <0.01 );compared with the SD0 group, the concentration of blood serum IL-6 in SD, , SD4 and SD7 group rise significant^ P <0. 01 ). The concentration of blood serum IL-6 tends to rise first and then decline as the sleep deprivation prolongs. Conclusion ( 1 )The expression of TNF-a and IL-6 in blood serum of the sleep deprivation SD rats were related with degree and duration of sleep deprivation. ( 2 )The rise in TNF-a and IL-6 caused by hypoxia, hypothermia and sleep deprivation might protect the brain cells in the short run,but long-term continued increase of its level would like to injure the neuron.关键词
低氧/低温/睡眠剥夺/TNF-α/IL-6/大鼠Key words
Hypoxia/ Hypothermia/ Sleep deprivation/ TNF-α/IL-6/Rats引用本文复制引用
刘诗翔,刘枢晓,孔繁一,周静..大鼠低氧低温睡眠剥夺后血清TNF-α和IL-6水平变化的研究[J].疑难病杂志,2011,10(12):914-916,3.基金项目
全军"十一五"重点资助课题(No.06MA141) （No.06MA141）
