中国肿瘤生物治疗杂志2011，Vol.18Issue(5)：508-513,6.DOI:10.3872/j.issn.1007-385X.2011.05.008
PTEN抑制人脐静脉内皮细胞ECV304的增殖和VEGF的表达
PTEN inhibits proliferation and VEGF expression in human umbilical vein endothelial ECV304 cells
摘要
Abstract
Objective: To investigate the effect of phosphatase and tensin hemology deleted on chromosome ten gene (PTEN) on proliferation, apoptosis, VEGF (vascular endothelial growth factor) and its receptor VEGFR1 expression in human umbilical vein endothelial cell (HUVEC) line ECV304. Methods; Recombinant adenovirus containing green fluorescent protein (GFP) and PTEN ( Ad-PTEN-GFP) or empty vector ( Ad-GFP) were transfected into ECV304 cells; proliferation and apoptosis of ECV304 cells were measured by MTT assay, Hoechst3342 staining and flow cytometry, respectively; PTEN, VEGF, VEGFR1 mRNA expression levels in Ad-PTEN-GFP-transfected ECV304 cells were examined by quantitative PCR; and VEGF protein level in ECV304 cell supernatant was detected by ELISA. Chick chorioallantoic membrane (CAM) assay was used to study the effect of PTEN on angiogenesis. Results; Ad-PTEN-GFP transfection significantly inhibited the proliferation and induced apoptosis of ECV304 cells and the inhibitory rate and apoptotic rate were (50.38 ±5.42)% and (73.3 ±5.3)% at 5 d. VEGF and VEGFR1 mRNA expression levels were (13.40 ± 1. 32)% and (46.12 ±5.20)% of untransfected group after transfected with Ad-PTEN-GFP at MOI = 100 in ECV304 cells. Furthermore, CAM assay results showed that Ad-PTEN-GFP transfection inhibited CAM angiogenesis in vivo. Conclusion:PTEN can inhibit the growth of and promote apoptosis of human umbilical vein endothelial ECV304 cells, which might berelated to the down-regulation of VEGF/VEGFR1 expression and the resulting angiogenesis inhibition.关键词
PTEN基因/血管内皮细胞生长因子(VEGF)/血管内皮细胞生长因子受体1(VEGFR1)/血管新生/ECV304细胞Key words
PTEN/ vascular endothelial growth factor( VEGF) / VEGF receptor( VEGFR1) / angiogenesis/ ECV304 cell分类
医药卫生引用本文复制引用
颜晓燕,成志勇,李琳,梁丽青,魏占惠,王亚丽,司淼,高连彬..PTEN抑制人脐静脉内皮细胞ECV304的增殖和VEGF的表达[J].中国肿瘤生物治疗杂志,2011,18(5):508-513,6.基金项目
保定市科技攻关计划资助项目(No.11ZF003). （No.11ZF003）
